Purification and characterization of a novel glucansucrase from Leuconostoc lactis EG001

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dc.contributor.authorY M Kim-
dc.contributor.authorM J Yeon-
dc.contributor.authorNack-Shick Choi-
dc.contributor.authorYoung Hyo Chang-
dc.contributor.authorMin-Young Jung-
dc.contributor.authorJae Jun Song-
dc.contributor.authorJoong Su Kim-
dc.date.accessioned2017-04-19T09:19:07Z-
dc.date.available2017-04-19T09:19:07Z-
dc.date.issued2010-
dc.identifier.issn0944-5013-
dc.identifier.uri10.1016/j.micres.2009.08.005ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9627-
dc.description.abstractA gene encoding glucansucrase was identified in Leuconostoc lactis EG001 isolated from lactic acid bacteria (LAB) in Kimchi, a traditional Korean fermented food. The L. lactis EG001 glucansucrase gene consists of 4503. bp open reading frame (ORF) and encodes an enzyme of 1500 amino acids with an apparent molecular mass of 165. kDa. The deduced amino-acid sequence showed the highest amino-acid sequence identity (75%) to that of dextransucrase of L. mesenteroides. The gene was cloned and over-expressed in Escherichia coli strain. The recombinant enzyme was purified via Ni-NTA affinity chromatography and its enzymatic properties were characterized. The enzyme exhibited optimum activity at 30°C and pH 5.0. In addition, the enzyme was able to catalyze the glycosylation of l-ascorbic acid to l-ascorbic acid 2-glucoside. The glycosylated product via EG001 glucansucrase has the potential as an antioxidant in industrial applications.-
dc.publisherElsevier-
dc.titlePurification and characterization of a novel glucansucrase from Leuconostoc lactis EG001-
dc.title.alternativePurification and characterization of a novel glucansucrase from Leuconostoc lactis EG001-
dc.typeArticle-
dc.citation.titleMicrobiological Research-
dc.citation.number5-
dc.citation.endPage391-
dc.citation.startPage384-
dc.citation.volume165-
dc.contributor.affiliatedAuthorNack-Shick Choi-
dc.contributor.affiliatedAuthorYoung Hyo Chang-
dc.contributor.affiliatedAuthorMin-Young Jung-
dc.contributor.affiliatedAuthorJae Jun Song-
dc.contributor.affiliatedAuthorJoong Su Kim-
dc.contributor.alternativeName김용모-
dc.contributor.alternativeName연민지-
dc.contributor.alternativeName최낙식-
dc.contributor.alternativeName장영효-
dc.contributor.alternativeName정민영-
dc.contributor.alternativeName송재준-
dc.contributor.alternativeName김중수-
dc.identifier.bibliographicCitationMicrobiological Research, vol. 165, no. 5, pp. 384-391-
dc.identifier.doi10.1016/j.micres.2009.08.005-
dc.subject.keywordGlucansucrase-
dc.subject.keywordGlycosylation-
dc.subject.keywordL-ascorbic acid-
dc.subject.keywordL-ascorbic acid 2-glucoside-
dc.subject.localGlucansucrase-
dc.subject.localglucansucrase-
dc.subject.localglycosylation-
dc.subject.localGlycosylation-
dc.subject.localL-ascorbic acid-
dc.subject.localL-ascorbic acid 2-glucoside-
dc.description.journalClassY-
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Bio-Infrastructure Policy Support Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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