Enhanced production of ethanol from glycerol by engineered Hansenula polymorpha expressing pyruvate decarboxylase and aldehyde dehydrogenase genes from Zymomonas mobilis = 재조합효모를 이용한 글리세롤로부터 에탄올 생산

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dc.contributor.authorWon Kyung Hong-
dc.contributor.authorChul Ho Kim-
dc.contributor.authorSun Yeon Heo-
dc.contributor.authorL H Luo-
dc.contributor.authorBaek Rock Oh-
dc.contributor.authorJeong Woo Seo-
dc.date.accessioned2017-04-19T09:19:07Z-
dc.date.available2017-04-19T09:19:07Z-
dc.date.issued2010-
dc.identifier.issn0141-5492-
dc.identifier.uri10.1007/s10529-010-0259-zko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9629-
dc.description.abstractTo improve production of ethanol from glycerol, the methylotrophic yeast Hansenula polymorpha was engineered to express the pdc and adhB genes encoding pyruvate decarboxylase and aldehyde dehydrogenase II from Zymomonas mobilis, respectively, under the control of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. The ethanol yield was 3.3-fold higher (2.74 g l-1) in the engineered yeast compared with the parent strain (0.83 g l-1). Further engineering to stimulate glycerol utilization in the recombinant strain via expression of dhaD and dhaKLM genes from Klebsiella pneumoniae encoding glycerol dehydrogenase and dehydroxyacetone kinase, respectively, resulted in a 3.7-fold increase (3.1 g l-1) in ethanol yield.-
dc.publisherSpringer-
dc.titleEnhanced production of ethanol from glycerol by engineered Hansenula polymorpha expressing pyruvate decarboxylase and aldehyde dehydrogenase genes from Zymomonas mobilis = 재조합효모를 이용한 글리세롤로부터 에탄올 생산-
dc.title.alternativeEnhanced production of ethanol from glycerol by engineered Hansenula polymorpha expressing pyruvate decarboxylase and aldehyde dehydrogenase genes from Zymomonas mobilis-
dc.typeArticle-
dc.citation.titleBiotechnology Letters-
dc.citation.number8-
dc.citation.endPage1082-
dc.citation.startPage1077-
dc.citation.volume32-
dc.contributor.affiliatedAuthorWon Kyung Hong-
dc.contributor.affiliatedAuthorChul Ho Kim-
dc.contributor.affiliatedAuthorSun Yeon Heo-
dc.contributor.affiliatedAuthorL H Luo-
dc.contributor.affiliatedAuthorBaek Rock Oh-
dc.contributor.affiliatedAuthorJeong Woo Seo-
dc.contributor.alternativeName홍원경-
dc.contributor.alternativeName김철호-
dc.contributor.alternativeName허선연-
dc.contributor.alternativeNameLuo-
dc.contributor.alternativeName오백록-
dc.contributor.alternativeName서정우-
dc.identifier.bibliographicCitationBiotechnology Letters, vol. 32, no. 8, pp. 1077-1082-
dc.identifier.doi10.1007/s10529-010-0259-z-
dc.subject.keywordAldehyde dehydrogenase II-
dc.subject.keywordEthanol production-
dc.subject.keywordGlycerol-
dc.subject.keywordHansenula polymorpha-
dc.subject.keywordPyruvate decarboxylase-
dc.subject.localAldehyde dehydrogenase II-
dc.subject.localEthanol production-
dc.subject.localethanol production-
dc.subject.localGlycerol-
dc.subject.localglycerol-
dc.subject.localHansenulapolymorpha-
dc.subject.localhansenula polymorpha-
dc.subject.localHansenula polymorpha-
dc.subject.localHansenula polymorpha (Pichia angusta)-
dc.subject.localPyruvate decarboxylase-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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