S. pombe genome deletion project: An update

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dc.contributor.authorM Spirek-
dc.contributor.authorZ Benko-
dc.contributor.authorM Carnecka-
dc.contributor.authorC Rumpf-
dc.contributor.authorL Cipak-
dc.contributor.authorM Batova-
dc.contributor.authorI Marova-
dc.contributor.authorM Nam-
dc.contributor.authorDong Uk Kim-
dc.contributor.authorH O Park-
dc.contributor.authorJ Hayles-
dc.contributor.authorKwang Lae Hoe-
dc.contributor.authorP Nurse-
dc.contributor.authorJ Gregan-
dc.date.accessioned2017-04-19T09:19:08Z-
dc.date.available2017-04-19T09:19:08Z-
dc.date.issued2010-
dc.identifier.issn1538-4101-
dc.identifier.uri10.4161/cc.9.12.11914ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9631-
dc.description.abstractThe fission yeast Schizosaccharomyces pombe is a model organism used widely to study various aspects of eukaryotic biology. A collection of heterozygous diploid strains containing individual deletions in nearly all S. pombe genes has been created using a PCR based strategy. However, deletion of some genes has not been possible using this methodology. Here we use an efficient knockout strategy based on plasmids that contain large regions homologous to the target gene to delete an additional 29 genes. The collection of deletion mutants now covers 99% of the fission yeast open reading frames.-
dc.publisherT&F (Taylor & Francis)-
dc.titleS. pombe genome deletion project: An update-
dc.title.alternativeS. pombe genome deletion project: An update-
dc.typeArticle-
dc.citation.titleCell Cycle-
dc.citation.number12-
dc.citation.endPage2402-
dc.citation.startPage2399-
dc.citation.volume9-
dc.contributor.affiliatedAuthorDong Uk Kim-
dc.contributor.affiliatedAuthorKwang Lae Hoe-
dc.contributor.alternativeNameSpirek-
dc.contributor.alternativeNameBenko-
dc.contributor.alternativeNameCarnecka-
dc.contributor.alternativeNameRumpf-
dc.contributor.alternativeNameCipak-
dc.contributor.alternativeNameBatova-
dc.contributor.alternativeNameMarova-
dc.contributor.alternativeName남미영-
dc.contributor.alternativeName김동욱-
dc.contributor.alternativeName박한오-
dc.contributor.alternativeNameHayles-
dc.contributor.alternativeName허광래-
dc.contributor.alternativeNameNurse-
dc.contributor.alternativeNameGregan-
dc.identifier.bibliographicCitationCell Cycle, vol. 9, no. 12, pp. 2399-2402-
dc.identifier.doi10.4161/cc.9.12.11914-
dc.subject.keywordEssential genes-
dc.subject.keywordFission yeast-
dc.subject.keywordGene deletion-
dc.subject.keywordGenomics-
dc.subject.keywordVectors-
dc.subject.localEssential genes-
dc.subject.localFission yeast-
dc.subject.localfission yeast-
dc.subject.localgene-deletion-
dc.subject.localGene deletion-
dc.subject.localgene deletion-
dc.subject.localGenomics-
dc.subject.localgenomics-
dc.subject.localvector-
dc.subject.localVectors-
dc.subject.localVector-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
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