Downregulation of immune response by the human cytokines Interleukin-32α and β in cell-mediated rejection

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Title
Downregulation of immune response by the human cytokines Interleukin-32α and β in cell-mediated rejection
Author(s)
J I Chae; J H Shim; K S Lee; Y S Cho; Kyu-Sun Lee; D Y Yoon; S H Kim; H M Chung; D B Koo; C S Park; D S Lee; P K Myung
Bibliographic Citation
Cellular Immunology, vol. 264, no. 1, pp. 47-53
Publication Year
2010
Abstract
Xenotransplantation of porcine organs has the potential to help overcome the severe shortage of human tissues and organs available for human transplantation. However, numerous hurdles such as immune-mediated xenograft rejection remain before clinical xenotransplantation. In this study, we elucidated the role of human TNF-α-inducing factor, Interleukin-32 (IL-32), in porcine kidney cells (PK-15) during cell-mediated rejection by examining host cell responses. CD8+ and CD4+ T cells numbers were reduced in the lymph nodes of PK-15/IL-32β injected mice. CD3+ Tcells were in mice injected with control cells but PK-15/IL-32α- and PK-15/IL-32β-injected cell numbers were lower in lymphnodes than un transfected controls. In Mixed lymphocyte reaction cultures, the rates of cell proliferation were increased in both PK-15/IL-32α- and PK-15/IL-32β-injected groups compared to the untransfected control groups. The Stable porcine PK-15 cells expression IL-32α and IL-32β inhibited cytotoxic T lymphocyte (CTLs) after cellular xenograft. Our results suggest that human IL-32α and IL-32β regulates on xenograft rejection in cellular xenotransplantation.
Keyword
CytokinesIL-32αIL-32βImmune systemPigXenograft rejectionXenotransplantation
ISSN
0008-8749
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.cellimm.2010.04.010
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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