Purification and characterization of OmcZ, an outer-surface, octaheme c-type cytochrome essential for optimal current production by Geobacter sulfurreducens

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dc.contributor.authorK Inoue-
dc.contributor.authorX Qian-
dc.contributor.authorL Morgado-
dc.contributor.authorByoung Chan Kim-
dc.contributor.authorM Izallalen-
dc.contributor.authorC A Salgueiro-
dc.contributor.authorD R Lovley-
dc.date.accessioned2017-04-19T09:19:09Z-
dc.date.available2017-04-19T09:19:09Z-
dc.date.issued2010-
dc.identifier.issn00992240-
dc.identifier.uri10.1128/AEM.00027-10ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9641-
dc.description.abstractPrevious studies have demonstrated that Geobacter sulfurreducens requires the c-type cytochrome OmcZ, which is present in large (OmcZL; 50-kDa) and small (OmcZs; 30-kDa) forms, for optimal current production in microbial fuel cells. This protein was further characterized to aid in understanding its role in current production. Subcellular-localization studies suggested that OmcZs was the predominant extracellular form of OmcZ. N- and C-terminal amino acid sequence analysis of purified OmcZs and molecular weight measurements indicated that OmcZs is a cleaved product of OmcZL retaining all 8 hemes, including 1 heme with the unusual c-type heme-binding motif CX14CH. The purified OmcZs was remarkably thermally stable (thermaldenaturing temperature, 94.2°C). Redox titration analysis revealed that the midpoint reduction potential of OmcZ s is approximately - 220 mV (versus the standard hydrogen electrode [SHE]) with nonequivalent heme groups that cover a large reduction potential range (- 420 to - 60 mV). OmcZs transferred electrons in vitro to a diversity of potential extracellular electron acceptors, such as Fe(III) citrate, U(VI), Cr(VI), Au(III), Mn(IV) oxide, and the humic substance analogue anthraquinone-2,6-disulfonate, but not Fe(III) oxide. The biochemical properties and extracellular localization of OmcZ suggest that it is well suited for promoting electron transfer in current-producing biofilms of G. sulfurreducens.-
dc.publisherAmer Soc Microb-
dc.titlePurification and characterization of OmcZ, an outer-surface, octaheme c-type cytochrome essential for optimal current production by Geobacter sulfurreducens-
dc.title.alternativePurification and characterization of OmcZ, an outer-surface, octaheme c-type cytochrome essential for optimal current production by Geobacter sulfurreducens-
dc.typeArticle-
dc.citation.titleApplied and Environmental Microbiology-
dc.citation.number12-
dc.citation.endPage4007-
dc.citation.startPage3999-
dc.citation.volume76-
dc.contributor.affiliatedAuthorByoung Chan Kim-
dc.contributor.alternativeNameInoue-
dc.contributor.alternativeNameQian-
dc.contributor.alternativeNameMorgado-
dc.contributor.alternativeName김병찬-
dc.contributor.alternativeNameIzallalen-
dc.contributor.alternativeNameSalgueiro-
dc.contributor.alternativeNameLovley-
dc.identifier.bibliographicCitationApplied and Environmental Microbiology, vol. 76, no. 12, pp. 3999-4007-
dc.identifier.doi10.1128/AEM.00027-10-
dc.description.journalClassY-
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Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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