Fast microchip electrophoresis using field strength gradients for single nucleotide polymorphism identification of cattle breeds

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dc.contributor.authorD Oh-
dc.contributor.authorI C Cheong-
dc.contributor.authorHee Gu Lee-
dc.contributor.authorS K Eo-
dc.contributor.authorS H Kang-
dc.date.accessioned2017-04-19T09:19:19Z-
dc.date.available2017-04-19T09:19:19Z-
dc.date.issued2010-
dc.identifier.issn0253-2964-
dc.identifier.uri10.5012/bkcs.2010.31.7.1902ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9648-
dc.description.abstractA microchip electrophoresis (ME) method was developed using a programmed field strength gradients (PFSG) for the single nucleotide polymorphism (SNP) based fast identification of cattle breeds. Four different Korean cattle (Hanwoo) and Holstein SNP markers amplified by allele-specific polymerase chain reaction were separated in a glass microchip filled with 0.5% poly(ethyleneoxide) (Mr = 8 000 000) by PFSG as follows: 750 V/cm for 0 - 14 s, 166.7 V/cm for 14 - 31 s, 83.3 V/cm for 31 - 46 s, and 750 V/cm for 46 - 100 s. The cattle breeds were clearly distinguished within 45 s. The ME-PFSG method was 7 times and 5 times faster than the constant electric field ME method and the capillary electrophoresis- PFSG method, respectively, with a high resolving power (Rs = 5.05 - 9.98). The proposed methodology could be a powerful tool for the fast and simultaneous determination of SNP markers for various cattle breeds with high accuracy.-
dc.publisherWiley-
dc.titleFast microchip electrophoresis using field strength gradients for single nucleotide polymorphism identification of cattle breeds-
dc.title.alternativeFast microchip electrophoresis using field strength gradients for single nucleotide polymorphism identification of cattle breeds-
dc.typeArticle-
dc.citation.titleBulletin of Korean Chemical Society-
dc.citation.number7-
dc.citation.endPage1906-
dc.citation.startPage1902-
dc.citation.volume31-
dc.contributor.affiliatedAuthorHee Gu Lee-
dc.contributor.alternativeName오두리-
dc.contributor.alternativeName정일청-
dc.contributor.alternativeName이희구-
dc.contributor.alternativeName어성국-
dc.contributor.alternativeName강성호-
dc.identifier.bibliographicCitationBulletin of Korean Chemical Society, vol. 31, no. 7, pp. 1902-1906-
dc.identifier.doi10.5012/bkcs.2010.31.7.1902-
dc.subject.keywordCattle breed-
dc.subject.keywordFast separation-
dc.subject.keywordField strength gradients-
dc.subject.keywordMicrochip gel electrophoresis-
dc.subject.keywordSingle nucleotide polymorphism (SNP)-
dc.subject.localCattle breed-
dc.subject.localFast separation-
dc.subject.localField strength gradients-
dc.subject.localMicrochip gel electrophoresis-
dc.subject.localSingle Nucleotide Polymorphism-
dc.subject.localsingle nucleotide polymorphism-
dc.subject.localsingle nucleotide polymorphism (SNP)-
dc.subject.localSingle nucleotide polymorphisms (SNPs)-
dc.subject.localSingle-nucleotide polymorphism-
dc.subject.localSingle nucleotide polymorphism-
dc.subject.localSingle nucleotide polymorphisms-
dc.subject.localsingle-nucleotide polymorphism-
dc.subject.localSingle nucleotide polymorphism (SNP)-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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