Diversity and abundance of ammonia-oxidizing bacteria in activated sludge treating different types of wastewater

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dc.contributor.authorK Baek-
dc.contributor.authorC Park-
dc.contributor.authorHee-Mock Oh-
dc.contributor.authorByung Dae Yoon-
dc.contributor.authorHee-Sik Kim-
dc.date.accessioned2017-04-19T09:19:22Z-
dc.date.available2017-04-19T09:19:22Z-
dc.date.issued2010-
dc.identifier.issn1017-7825-
dc.identifier.uri10.4014/jmb.0907.07021ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9663-
dc.description.abstractThe diversity and abundance of ammonia-oxidizing bacteria (AOB) in activated sludge were compared using PCR- DGGE and real-time PCR assays. Activated sludge samples were collected from five different types of wastewater treatment plants (WWTPs) mainly treating textile, paper, food, and livestock wastewater or domestic sewage. The composition of total bacteria determined by PCR-DGGE was highly diverse between the samples, whereas the community of AOB was similar across all the investigated activated sludge. Total bacterial numbers and AOB numbers in the aerated mixed liquor were in the range of 1.8x1010 to 3.8x1012 and 1.7x106 to 2.7x1010 copies/l, respectively. Activated sludge from livestock, textile, and sewage treating WWTPs contained relatively high amoA gene copies (more than 105 copies/l), whereas activated sludge from food and paper WWTPs revealed a low number of the amoA gene (less than 103 copies/l). The value of the amoA gene copy effectively showed the difference in composition of bacteria in different activated sludge samples and this was better than the measurement with the AOB 16S rRNA or total 16S rRNA gene. These results suggest that the quantification of the amoA gene can help monitor AOB and ammonia oxidation in WWTPs.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleDiversity and abundance of ammonia-oxidizing bacteria in activated sludge treating different types of wastewater-
dc.title.alternativeDiversity and abundance of ammonia-oxidizing bacteria in activated sludge treating different types of wastewater-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number7-
dc.citation.endPage1133-
dc.citation.startPage1128-
dc.citation.volume20-
dc.contributor.affiliatedAuthorHee-Mock Oh-
dc.contributor.affiliatedAuthorByung Dae Yoon-
dc.contributor.affiliatedAuthorHee-Sik Kim-
dc.contributor.alternativeName백경화-
dc.contributor.alternativeName박철-
dc.contributor.alternativeName오희목-
dc.contributor.alternativeName윤병대-
dc.contributor.alternativeName김희식-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 20, no. 7, pp. 1128-1133-
dc.identifier.doi10.4014/jmb.0907.07021-
dc.subject.keywordActivated sludge-
dc.subject.keywordAmmonia-oxidizing bacteria-
dc.subject.keywordPCR-DGGE-
dc.subject.keywordReal-time PCR-
dc.subject.keywordWastewater-
dc.subject.localActivated sludge-
dc.subject.localactivated sludge-
dc.subject.localAmmonia-oxidizing bacteria-
dc.subject.localPCR-DGGE-
dc.subject.localReal-time PCR-
dc.subject.localreal time PCR-
dc.subject.localReal time PCR-
dc.subject.localreal-time PCR-
dc.subject.localwastewater-
dc.subject.localWastewater-
dc.description.journalClassY-
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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