Short rare hTERT-VNTR2-2nd alleles are associated with prostate cancer susceptibility and influence gene expression

Cited 11 time in scopus
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Title
Short rare hTERT-VNTR2-2nd alleles are associated with prostate cancer susceptibility and influence gene expression
Author(s)
S L Yoon; S I Jung; D J Do; S R Lee; Sang-Yeop Lee; In-Sun Chu; W J Kim; J Jung; C S Kim; S H Cheon; S H Leem
Bibliographic Citation
BMC Cancer, vol. 10, pp. 393-393
Publication Year
2010
Abstract
Background: The hTERT (human telomerase reverse transcriptase) gene contains five variable number tandem repeats (VNTR) and previous studies have described polymorphisms for hTERT-VNTR2-2nd. We investigated how allelic variation in hTERT-VNTR2-2ndmay affect susceptibility to prostate cancer.Methods: A case-control study was performed using DNA from 421 cancer-free male controls and 329 patients with prostate cancer. In addition, to determine whether the VNTR polymorphisms have a functional consequence, we examined the transcriptional levels of a reporter gene linked to these VNTRs and driven by the hTERT promoter in cell lines.Results: Three new rare alleles were detected from this study, two of which were identified only in cancer subjects. A statistically significant association between rare hTERT-VNTR2-2ndalleles and risk of prostate cancer was observed [OR, 5.17; 95% confidence interval (CI), 1.09-24.43; P = 0.021]. Furthermore, the results indicated that these VNTRs inserted in the enhancer region could influence the expression of hTERT in prostate cancer cell lines.Conclusions: This is the first study to report that rare hTERT VNTRs are associated with prostate cancer predisposition and that the VNTRs can induce enhanced levels of hTERT promoter activity in prostate cancer cell lines. Thus, the hTERT-VNTR2-2ndlocus may function as a modifier of prostate cancer risk by affecting gene expression.
ISSN
1471-2407
Publisher
Springer-BMC
DOI
http://dx.doi.org/10.1186/1471-2407-10-393
Type
Article
Appears in Collections:
Division of Biomedical Research > Genome Editing Research Center > 1. Journal Articles
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