L1 cell adhesion molecule is a novel therapeutic target in intrahepatic cholangiocarcinoma

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dc.contributor.authorJeong Ki Min-
dc.contributor.authorJ M Kim-
dc.contributor.authorS Li-
dc.contributor.authorJ W Lee-
dc.contributor.authorHyunho Yoon-
dc.contributor.authorC J Ryu-
dc.contributor.authorS H Jeon-
dc.contributor.authorJ H Lee-
dc.contributor.authorJ Y Kim-
dc.contributor.authorH K Yoon-
dc.contributor.authorY K Lee-
dc.contributor.authorBong-Hui Kim-
dc.contributor.authorYeon Sung Son-
dc.contributor.authorH S Choi-
dc.contributor.authorN K Lim-
dc.contributor.authorD G Kim-
dc.contributor.authorH J Hong-
dc.date.accessioned2017-04-19T09:19:27Z-
dc.date.available2017-04-19T09:19:27Z-
dc.date.issued2010-
dc.identifier.issn1078-0432-
dc.identifier.uri10.1158/1078-0432.CCR-09-3075ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9673-
dc.description.abstractPurpose: Intrahepatic cholangiocarcinoma (ICC), a highly malignant hepatobiliary cancer, has a poor prognosis and is refractory to conventional therapies. The aim of this study is to discover a novel molecular target for the treatment of ICC. Experimental Design: To discover novel cancer-associated membrane antigens expressed in ICC cells, we generated monoclonal antibodies (mAb) by immunizing mice with intact ICC cell lines and screened for those that bind to the plasma membrane of ICC cells but not to normal cells. The mAb A10-A3 was selected and its target antigen was identified as the L1 cell adhesion molecule. Expression of L1 in ICC was evaluated by immunohistochemical analysis of tumor samples from 42 ICC patients. The functional significance of L1 expression in the tumor progression of ICC was investigated by L1 suppression, L1 overexpression, and antibody treatment. Results: L1 was not expressed in normal hepatocytes and intrahepatic bile duct epithelium but highly expressed in 40.5% of ICC patients, remarkably at the invasive front of the tumors. Suppression of L1 with short hairpin RNA significantly decreased proliferation, migration, and invasion of ICC cells in vitro. Consistently, L1 overexpression in ICC cells enhanced proliferation, migration, invasion, and apoptosis resistance. In addition, L1 short hairpin RNA or anti-L1 mAb significantly reduced the tumor growth in nude mice bearing ICC xenograft. Conclusions: We identified that L1 is expressed in ICC. L1 plays an important role in the tumor progression of ICC by enhancing cell proliferation, migration, invasion, and survival. L1 may represent a novel therapeutic target for ICC.-
dc.publisherAmer Assoc Cancer Research-
dc.titleL1 cell adhesion molecule is a novel therapeutic target in intrahepatic cholangiocarcinoma-
dc.title.alternativeL1 cell adhesion molecule is a novel therapeutic target in intrahepatic cholangiocarcinoma-
dc.typeArticle-
dc.citation.titleClinical Cancer Research-
dc.citation.number14-
dc.citation.endPage3580-
dc.citation.startPage3571-
dc.citation.volume16-
dc.contributor.affiliatedAuthorJeong Ki Min-
dc.contributor.affiliatedAuthorHyunho Yoon-
dc.contributor.affiliatedAuthorBong-Hui Kim-
dc.contributor.affiliatedAuthorYeon Sung Son-
dc.contributor.alternativeName민정기-
dc.contributor.alternativeName김진만-
dc.contributor.alternativeName이성진-
dc.contributor.alternativeName이정회-
dc.contributor.alternativeName윤현호-
dc.contributor.alternativeName류춘제-
dc.contributor.alternativeName전성후-
dc.contributor.alternativeName이재혁-
dc.contributor.alternativeName김진영-
dc.contributor.alternativeName윤희관-
dc.contributor.alternativeName이영관-
dc.contributor.alternativeName김봉휘-
dc.contributor.alternativeName손연성-
dc.contributor.alternativeName최홍서-
dc.contributor.alternativeName임남규-
dc.contributor.alternativeName김대건-
dc.contributor.alternativeName홍효정-
dc.identifier.bibliographicCitationClinical Cancer Research, vol. 16, no. 14, pp. 3571-3580-
dc.identifier.doi10.1158/1078-0432.CCR-09-3075-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
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