Development of a stationary phase-specific autoinducible expression system in Bacillus subtilis = 고초균에서 자동유도 발현시스템 개발

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Title
Development of a stationary phase-specific autoinducible expression system in Bacillus subtilis = 고초균에서 자동유도 발현시스템 개발
Author(s)
S J Lee; Jae Gu Pan; Seung Hwan Park; Soo Keun Choi
Bibliographic Citation
Journal of Biotechnology, vol. 149, no. 1, pp. 16-20
Publication Year
2010
Abstract
Bacillus thuringiensis produces crystal proteins (Cry) that account for up to 25% of the dry cell weight during the stationary phase. The high-level expression and stationary phase-specific autoinduction of the cry gene led to development of a cry promoter-based Bacillus expression system. Among the various cry promoters, cry3Aa promoter was selected by comparing the lacZ expression levels in Bacillus subtilis. An extracellular enzyme cellulase was highly upregulated during the stationary phase while under control of the cry3Aa promoter. Improvement of the cry3Aa promoter was obtained by modification of the promoter sequence. Specifically, a 5-fold increase in lacZ expression was obtained by changing both the -35 and -10 boxes of the cry3Aa promoter to the consensus sequence of the σA-dependent promoter of B. subtilis. The modified cry3Aa promoter produced a significantly higher yield of AprE, which suggests that the promoter may be useful for high-level protein expression in B. subtilis.
Keyword
Bacillus subtilisBacillus thuringiensis cry3AaExpression systemPromoter modification
ISSN
0168-1656
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.jbiotec.2010.06.021
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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