Generation of donor natural killer cells from CD34 progenitor cells and subsequent infusion after HLA-mismatched allogeneic hematopoietic cell transplantation: A feasibility study

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Title
Generation of donor natural killer cells from CD34 progenitor cells and subsequent infusion after HLA-mismatched allogeneic hematopoietic cell transplantation: A feasibility study
Author(s)
Suk Ran Yoon; Y S Lee; S H Yang; K H Ahn; J H Lee; J H Lee; D Y Kim; Y A Kang; M Jeon; M Seol; S G Ryu; J W Chung; In Pyo Choi; K H Lee
Bibliographic Citation
Bone Marrow Transplantation, vol. 45, no. 6, pp. 1038-1046
Publication Year
2010
Abstract
Post transplant infusion of donor-type natural killer (NK) cells has been shown to have an anti-leukemia-enhancing effect without evoking GVHD in murine hematopoietic cell transplantation (HCT) models. Here, we tested 14 patients (age, 23-65 years), 12 with acute leukemia and 2 with myelodysplastic syndrome, who underwent HLA-mismatched HCT and subsequently received donor NK cell infusions. Cell donors (age, 16-51 years), comprising seven siblings, five offspring, and two mothers of the patients, underwent growth factor-mobilized leukapheresis for 3-5 days. Cells collected on the first 2-4 days were used for HCT, whereas those collected on the last day were CD34+ selected by magnetic-activated cell sorting (median, 2.22 × 106 cells/kg; range, 0.29-5.66). Donor NK cells were generated from the CD34+ cells by ex vivo cell culture over a 6-week period (median, 9.28 × 10 6 cells/kg; range, 0.33-24.50; CD122/CD56+ 64%; CD3 1.0%; and viability 88%). There were no signs of acute toxicity in patients infused with these cells 6-7 weeks post transplant. Overall, one and five patients developed acute and chronic GVHD during post transplant period, respectively. These results showed that clinical-grade donor NK cell production from CD34 + cells is feasible.
Keyword
donor CD34+ cellsdonor NK cell infusionGCSFHLA-mismatched HCT
ISSN
0268-3369
Publisher
Springer-Nature Pub Group
DOI
http://dx.doi.org/10.1038/bmt.2009.304
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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