Isolation and functional analysis of cDNAs similar to Hyp-1 involved in hypericin biosynthesis from Hypericum erectum

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Title
Isolation and functional analysis of cDNAs similar to Hyp-1 involved in hypericin biosynthesis from Hypericum erectum
Author(s)
M L Jin; J C Ahn; B Hwang; Hong-Seog Park; H S Lee; D W Choi
Bibliographic Citation
Biologia Plantarum, vol. 54, no. 4, pp. 725-729
Publication Year
2010
Abstract
Hypericin, a naphthodianthrone, has been identified as the principal active compound found in St. John's wort (Hypericum perforatum L.). To generate a gene resource for hypericin and other valuable metabolites, we generated expressed sequence tags (ESTs) from H. erectum. Analyses of the ESTs enabled us to select three cDNAs, HeHyp1, HeHyp2, and HeHyp3, evidencing significant sequence homology to Hyp-1 that were involved in hypericin biosynthesis from H. erectum. The deduced amino acid sequence of HeHyp1 cDNA exhibits 95 % identity with Hyp-1. The HeHyp2 and HeHyp3 polypeptides also exhibit 81.1 % identity with Hyp-1. The transcripts of HeHyp1, HeHyp2, and HeHyp3 were detected in the root, stem, leaf, flower, and callus cells. Study using recombinant protein suggests that Hyp-1, HeHyp2, and HeHyp3 may be involved in the biosynthetic of hypericin or other emodin derivatives.
Keyword
amino acid sequenceESTsHPLCRT-PCRTLC
ISSN
0006-3134
Publisher
Acad Sciences Czech Republic, Inst Experimental Botany
DOI
http://dx.doi.org/10.1007/s10535-010-0129-5
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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