Expression and immunogenicity of a recombinant chimeric protein of human colorectal cancer antigen GA733-2 and an Fc antibody fragment in stably transformed Drosophila melanogaster S2 cells

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dc.contributor.authorY J Seok-
dc.contributor.authorK I Kim-
dc.contributor.authorK H Yoo-
dc.contributor.authorJ Hwang-Bo-
dc.contributor.authorH H Lee-
dc.contributor.authorD H Shon-
dc.contributor.authorK S Ko-
dc.contributor.authorH S Kang-
dc.contributor.authorK J Lee-
dc.contributor.authorDoo-Byoung Oh-
dc.contributor.authorY H Joung-
dc.contributor.authorI S Chung-
dc.date.accessioned2017-04-19T09:19:32Z-
dc.date.available2017-04-19T09:19:32Z-
dc.date.issued2010-
dc.identifier.issn0273-2289-
dc.identifier.uri10.1007/s12010-010-8909-0ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9705-
dc.description.abstractHuman colorectal cancer antigen GA733-2 fused to the immunoglobin Fc fragment (GA733-2-Fc) was expressed in stably transformed Drosophila melanogaster S2 cells, and the immunogenicity of recombinant GA733-2-Fc was investigated in mice. Recombinant GA733-2-Fc was secreted into a culture medium with a molecular mass of approximately 65 kDa. Recombinant GA733-2-Fc was purified to homogeneity using affinity fractionation with Protein A sepharose 4 Fast Flow. Recombinant GA733-2-Fc proteins elicited production of specific antibodies against recombinant GA733-2 by immunization through an intraperitoneal route. Recombinant GA733-2-Fc-induced antibodies showed a binding activity to human colorectal carcinoma HCT-116 cells. Secretory recombinant GA733-2-Fc from Drosophila S2 cell systems can be used as an effective experimental antigen for research in cancer vaccine development.-
dc.publisherSpringer-
dc.titleExpression and immunogenicity of a recombinant chimeric protein of human colorectal cancer antigen GA733-2 and an Fc antibody fragment in stably transformed Drosophila melanogaster S2 cells-
dc.title.alternativeExpression and immunogenicity of a recombinant chimeric protein of human colorectal cancer antigen GA733-2 and an Fc antibody fragment in stably transformed Drosophila melanogaster S2 cells-
dc.typeArticle-
dc.citation.titleApplied Biochemistry and Biotechnology-
dc.citation.number5-
dc.citation.endPage1445-
dc.citation.startPage1435-
dc.citation.volume162-
dc.contributor.affiliatedAuthorDoo-Byoung Oh-
dc.contributor.alternativeName-
dc.contributor.alternativeName-
dc.contributor.alternativeName-
dc.contributor.alternativeName황보-
dc.contributor.alternativeName-
dc.contributor.alternativeName-
dc.contributor.alternativeName-
dc.contributor.alternativeName-
dc.contributor.alternativeName-
dc.contributor.alternativeName오두병-
dc.contributor.alternativeName-
dc.contributor.alternativeName-
dc.identifier.bibliographicCitationApplied Biochemistry and Biotechnology, vol. 162, no. 5, pp. 1435-1445-
dc.identifier.doi10.1007/s12010-010-8909-0-
dc.subject.keywordCancer antigen-
dc.subject.keywordDrosophila melanogaster S2 cells-
dc.subject.keywordExpression-
dc.subject.keywordImmunogenicity-
dc.subject.keywordRecombinant GA733-2-Fc-
dc.subject.localCancer antigen-
dc.subject.localDrosophila melanogaster S2 cells-
dc.subject.localexpression-
dc.subject.localExpression-
dc.subject.localImmunogenicity-
dc.subject.localimmunogenicity-
dc.subject.localRecombinant GA733-2-Fc-
dc.description.journalClassY-
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