Down-modulation of Bis sensitizes cell death in C6 glioma cells induced by oxygen-glucose deprivation

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Title
Down-modulation of Bis sensitizes cell death in C6 glioma cells induced by oxygen-glucose deprivation
Author(s)
S E Jung; Y K Kim; D Y Youn; M H Lim; Jeong Heon Ko; Y S Ahn; J H Lee
Bibliographic Citation
Brain Research, vol. 1349, no. C, pp. 1-10
Publication Year
2010
Abstract
Bcl-2 interacting cell death suppressor (Bis), also known as Bag3, has been implicated in anti-stress and anti-apoptotic pathways. In a previous study, we observed a significant induction of Bis in reactive astrocytes of the rat hippocampus after transient forebrain ischemia. To investigate the significance of this induction in ischemic injury, the expression of Bis was reduced with siRNA in C6 glioma cells and exposed to oxygen-glucose deprivation (OGD) conditions. Bis knock-down resulted in an increase in the cell death rate of the C6 cells after OGD, accompanied by accumulation of reactive oxygen species. Among the cellular antioxidants, the induction of superoxide dismutase (SOD) activity was significantly interfered within the cells treated with bis siRNA treated cells (bis-kd C6). A Western blot assay revealed that SOD1 expression gradually increased in control cells, which was not observed in bis-kd cells upon OGD treatment. A quantitative analysis of Sod1 and Sod2 transcripts indicated that the induction of Sod1 was more evidently suppressed by the reduction of Bis. As a transcription factor candidate for the Sod1 gene, the activity of NF-κB was determined the nuclear translocation of p65, showing that the activation of NF-κB was attenuated in bis-kd C6. Supporting this, an overexpression of Bis augments the activation of NF-κB and Sod1 mRNA with an increased cell survival under OGD conditions. These results suggest that one of physiological significances of Bis induction in reactive astrocytes after ischemia in vivo is to protect glial cells from oxidative stress, probably via the induction of SOD1, which is related to the activation of NF-κB.
Keyword
BisC6NF-κBOxygen-glucose deprivationSuperoxide dismutase
ISSN
0006-8993
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.brainres.2010.06.043
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Genome Editing Research Center > 1. Journal Articles
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