Human papillomavirus type 16 E6-specific antitumor immunity is induced by oral administration of HPV16 E6-expressing Lactobacillus casei in C57BL/6 mice

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dc.contributor.authorTae Young Lee-
dc.contributor.authorY H Kim-
dc.contributor.authorK S Lee-
dc.contributor.authorJeong Ki Kim-
dc.contributor.authorI H Lee-
dc.contributor.authorJ M Yang-
dc.contributor.authorM H Sung-
dc.contributor.authorJ S Park-
dc.contributor.authorHaryoung Poo-
dc.date.accessioned2017-04-19T09:19:34Z-
dc.date.available2017-04-19T09:19:34Z-
dc.date.issued2010-
dc.identifier.citationCancer Immunology, Immunotherapy ,59,11,1727,1737ko
dc.identifier.issn0340-7004-
dc.identifier.uri10.1007/s00262-010-0903-4ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9714-
dc.description.abstractGiven that local cell-mediated immunity (CMI) against the human papillomavirus type 16 E6 (HPV16 E6) protein is important for eradication of HPV16 E6-expressing cancer cells in the cervical mucosa, the HPV16 E6 protein may be a target for the mucosal immunotherapy of cervical cancer. Here, we expressed the HPV16 E6 antigen on Lactobacillus casei (L. casei) and investigated E6-specific CMI following oral administration of the L. casei-PgsA-E6 to mice. Surface expression of HPV16 E6 antigens was confirmed and mice were orally inoculated with the L. casei-PgsA or the L. casei-PgsA-E6. Compared to the L. casei-PgsA-treated mice, significantly higher levels of serum IgG and mucosal IgA were observed in L. casei-PgsA-E6-immunized mice; these differences were significantly enhanced after boost. Consistent with this, systemic and local CMI were significantly increased after the boost, as shown by increased counts of IFN-γ-secreting cells in splenocytes, mesenteric lymph nodes (MLN), and vaginal samples. Furthermore, in the TC-1 tumor model, animals receiving the orally administered L. casei-PgsA-E6 showed reduced tumor size and increased survival rate versus mice receiving control (L. casei-PgsA) immunization. We also found that L. casei-PgsA-E6-induced antitumor effect was decreased by in vivo depletion of CD4+ or CD8+ T cells. Collectively, these results indicate that the oral administration of lactobacilli bearing the surface-displayed E6 protein induces T cell-mediated cellular immunity and antitumor effects in mice.-
dc.publisherSpringer-
dc.titleHuman papillomavirus type 16 E6-specific antitumor immunity is induced by oral administration of HPV16 E6-expressing Lactobacillus casei in C57BL/6 mice-
dc.title.alternativeHuman papillomavirus type 16 E6-specific antitumor immunity is induced by oral administration of HPV16 E6-expressing Lactobacillus casei in C57BL/6 mice-
dc.typeArticle-
dc.citation.titleCancer Immunology Immunotherapy-
dc.citation.number11-
dc.citation.endPage1737-
dc.citation.startPage1727-
dc.citation.volume59-
dc.contributor.affiliatedAuthorTae Young Lee-
dc.contributor.affiliatedAuthorJeong Ki Kim-
dc.contributor.affiliatedAuthorHaryoung Poo-
dc.contributor.alternativeName이태영-
dc.contributor.alternativeName김양현-
dc.contributor.alternativeName이경수-
dc.contributor.alternativeName김정기-
dc.contributor.alternativeName이일한-
dc.contributor.alternativeName양재명-
dc.contributor.alternativeName성문희-
dc.contributor.alternativeName박종섭-
dc.contributor.alternativeName부하령-
dc.identifier.bibliographicCitationCancer Immunology Immunotherapy, vol. 59, no. 11, pp. 1727-1737-
dc.identifier.doi10.1007/s00262-010-0903-4-
dc.subject.keywordAntitumor effect-
dc.subject.keywordCell-mediated immunity-
dc.subject.keywordHPV16 E6-
dc.subject.keywordLactobacillus casei-
dc.subject.keywordPgsA-
dc.subject.localAnti-tumor effects-
dc.subject.localAntitumor effects-
dc.subject.localantitumor effect-
dc.subject.localAnti-tumor effect-
dc.subject.localantitumor effects-
dc.subject.localAntitumor effect-
dc.subject.localCell-mediated immunity-
dc.subject.localcell-mediated immunity-
dc.subject.localHPV 16 E6-
dc.subject.localHPV16 E6-
dc.subject.localLactobacillus casei-
dc.subject.localPgsA-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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