DC Field | Value | Language |
---|---|---|
dc.contributor.author | S H Choi | - |
dc.contributor.author | T Y Kim | - |
dc.contributor.author | Sung Goo Park | - |
dc.contributor.author | G H Cha | - |
dc.contributor.author | D W Shin | - |
dc.contributor.author | J Y Chai | - |
dc.contributor.author | Y H Lee | - |
dc.date.accessioned | 2017-04-19T09:19:39Z | - |
dc.date.available | 2017-04-19T09:19:39Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0023-4001 | - |
dc.identifier.uri | 10.3347/kjp.2010.48.3.195 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/9722 | - |
dc.description.abstract | We studied on the proteomic characteristics of Toxoplasma gondii KI-1 tachyzoites which were originally isolated from a Korean patient, and compared with those of the well-known virulent RH strain using 2-dimensional electrophoresis (2-DE), mass spectrometry, and quantitative real-time PCR. Two-dimensional separation of the total proteins isolated from KI-1 tachyzoites revealed up to 150 spots, of which 121 were consistent with those of RH tachyzoites. of the remaining 29 spots, 14 showed greater than 5-fold difference in density between the KI-1 and RH tachyzoites at a pH of 5.0-8.0. Among the 14 spots, 5 from the KI-1 isolate and 7 from the RH strain were identified using MALDI-TOF mass spectrometry and database searches. The spots from the KI-1 tachyzoites were dense granule proteins (GRA 2, 3, 6, and 7), hypoxanthine- guanine-xanthine phosphoribosyltransferase (HGRPTase), and uracil phosphoribosyltransferase (UPRTase). The spots from the RH strain were surface antigen 1 (SAG 1), L-lactate dehydrogenase (LDH), actin, chorismate synthase, peroximal catalase, hexokinase, bifunctional dihydrofolate reductase-thymidylate synthase (DHTR-TS), and nucleosidetriphosphatases (NTPases). Quantitative real-time PCR supported our mass spectrometric results by showing the elevated expression of the genes encoding GRA 2, 3, and 6 and UPRTase in the KI-1 tachyzoites and those encoding GRA 7, SAG 1, NTPase, and chorismate synthase in the RH tachyzoites. These observations demonstrate that the protein compositions of KI-1 and RH tachyzoites are similar but differential protein expression is involved in virulence. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Proteomic analysis of Toxoplasma gondii KI-1 tachyzoites | - |
dc.title.alternative | Proteomic analysis of Toxoplasma gondii KI-1 tachyzoites | - |
dc.type | Article | - |
dc.citation.title | Korean Journal of Parasitology | - |
dc.citation.number | 3 | - |
dc.citation.endPage | 201 | - |
dc.citation.startPage | 195 | - |
dc.citation.volume | 48 | - |
dc.contributor.affiliatedAuthor | Sung Goo Park | - |
dc.contributor.alternativeName | 최시환 | - |
dc.contributor.alternativeName | 김태윤 | - |
dc.contributor.alternativeName | 박성구 | - |
dc.contributor.alternativeName | 차광호 | - |
dc.contributor.alternativeName | 신대환 | - |
dc.contributor.alternativeName | 채종일 | - |
dc.contributor.alternativeName | 이영하 | - |
dc.identifier.bibliographicCitation | Korean Journal of Parasitology, vol. 48, no. 3, pp. 195-201 | - |
dc.identifier.doi | 10.3347/kjp.2010.48.3.195 | - |
dc.subject.keyword | Korean isolate-1 (ki-1) | - |
dc.subject.keyword | Proteomics | - |
dc.subject.keyword | Quantitative real time pcr | - |
dc.subject.keyword | Toxoplasma gondii | - |
dc.subject.local | Korean isolate-1 (ki-1) | - |
dc.subject.local | Proteomic | - |
dc.subject.local | Proteomics | - |
dc.subject.local | Quantitative real time pcr | - |
dc.subject.local | Toxoplasma gondii | - |
dc.description.journalClass | Y | - |
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