Evaluating the persistence of DNA from decomposing transgenic watermelon tissues in the field = 형질전환 수박 DNA의 지속성 평가

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Title
Evaluating the persistence of DNA from decomposing transgenic watermelon tissues in the field = 형질전환 수박 DNA의 지속성 평가
Author(s)
B Lee; Ji-Young Park; Kee Woong Park; C H Harn; Hwan Mook Kim; Chang-Gi Kim
Bibliographic Citation
Journal of Plant Biology, vol. 53, no. 5, pp. 338-343
Publication Year
2010
Abstract
To analyze the persistence of the 35S promoter, nos terminator, and hpt, we buried the leaves and rootstocks of transgenic watermelons (Citrullus lanatus (Thunb.) Matsum. & Nakai) in 10 cm of soil. Qualitative and quantitative PCR analyses showed that the amount of transgenes in leaf samples was greatly decreased, by 70%, after 1 month, and only 2.5% remained after 2 months. No transgenes were detected in the leaves after 3 months. For buried rootstock samples, transgenes also degraded quickly, but a very small amount was still detectable up to 3 months later. In our investigation of possible gene transfer from decomposing transgenic watermelon to soil bacteria, only the 35S promoter was detected. However, further examination using colony dot hybridization tests indicated that such a transfer did not occur.
Keyword
Citrullus lanatusDNA persistenceSoilTransgenic cropWatermelon
ISSN
I000-0094
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s12374-010-9121-z
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > Bio-Evaluation Center > 1. Journal Articles
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