Receptor activator of nuclear factor {kappa}B ligand is a novel inducer of tissue factor in macrophages = RANKL은 대식세포에서 Tissue factor의 새로운 Inducer

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Title
Receptor activator of nuclear factor {kappa}B ligand is a novel inducer of tissue factor in macrophages = RANKL은 대식세포에서 Tissue factor의 새로운 Inducer
Author(s)
J Kim; Jeong Ki Min; J A Park; H J Doh; Y S Choi; J Rho; Y M Kim; Y G Kwon
Bibliographic Citation
Circulation Research, vol. 107, no. 7, pp. 871-876
Publication Year
2010
Abstract
Rationale: Although recent studies have suggested a role for the receptor activator of nuclear factor κB ligand (RANKL) in the late stages of atherosclerosis (eg, plaque destabilization and rupture), the underlying mechanisms and subsequent events are unclear. Objective: Because blood clotting is common after plaque rupture, we hypothesized that RANKL influenced tissue factor (TF) expression and activity to initiate the coagulation cascade. Methods and Results: RANKL increased the TF mRNA level and procoagulant activity in macrophages, as determined by semiquantitative reverse transcription polymerase chain reaction (semiquantitative RT-PCR) and a chromogenic assay. TF promoter analysis revealed that AP-1 and Egr-1 are responsible for RANKL-induced TF transcription. In addition, RANKL increased phosphorylation of c-Jun NH 2-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK)1/2. RANKL-induced TF expression was attenuated by JNK- and MEK1-specific inhibitors and by small interfering RNA knockdown of c-Jun and Egr-1. Conclusion: Our results indicate that RANKL induces TF in macrophages mainly through the cooperative action of AP-1 and Egr-1 via JNK and ERK1/2 pathways. These findings provide strong mechanistic support for the role of RANKL in the thrombogenicity of atherosclerotic plaques.
Keyword
atherosclerosisreceptor activator of nuclear factor-κB ligandthrombosistissue factor
ISSN
0009-7330
Publisher
Kluwer
DOI
http://dx.doi.org/10.1161/CIRCRESAHA.110.221168
Type
Article
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
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