Molecular switching fluorescence based high sensitive detection of label-free C-reactive protein on biochip

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Title
Molecular switching fluorescence based high sensitive detection of label-free C-reactive protein on biochip
Author(s)
S Islam; H Yu; Hee Gu Lee; S H Kang
Bibliographic Citation
Biosensors & Bioelectronics, vol. 26, no. 3, pp. 1028-1035
Publication Year
2010
Abstract
A novel detection technique on biochip for the quantification of label-free C-reactive protein (CRP) based on molecular switching of fluorescence (MSF) is demonstrated by total internal reflection fluorescence microscopy. It alters fluorescence intensity of fluoreseinamine isomer 1 (FAI) upon binding with its specific ligand, O-phosphorylethanolamine (PEA). In the MSF-based detection, FAI was used as an ink, printed on a 3-glycidoxypropyl-trimethoxysilane (GPTS)-coated glass coverslip. With the addition of GPTS conjugated PEA solution to the FAI-printed coverslip, the fluorescence intensity was remarkably decreased. Addition of CRP increased fluorescence intensity linearly in the range of 800. aM to 500. fM (R=0.997). The MSF-based biochip assay for the estimation of CRP in human sera showed ~200 times increased detection sensitivity in less than a third of the time to obtain results using a conventional enzyme-linked immunosorbent assay. This biochip detection is a promising new technique for the quantification of CRP molecules from trace amounts of clinical samples
Keyword
BiochipC-reactive protein (CRP)Label-free detectionMolecular switching fluorescenceTotal internal reflection fluorescence microscopy (TIRFM)
ISSN
0956-5663
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bios.2010.08.045
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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