Random breakup of microdroplets for single-cell encapsulation

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dc.contributor.authorE Um-
dc.contributor.authorSeung Goo Lee-
dc.contributor.authorJ K Park-
dc.date.accessioned2017-04-19T09:19:51Z-
dc.date.available2017-04-19T09:19:51Z-
dc.date.issued2010-
dc.identifier.issn0003-6951-
dc.identifier.uri10.1063/1.3500980ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9761-
dc.description.abstractMicrofluidic droplet-based technology enables encapsulation of cells in the isolated aqueous chambers surrounded by immiscible fluid but single-cell encapsulation efficiency is usually less than 30%. In this letter, we introduce a simple microgroove structure to break droplets into random sizes which further allows collecting of single-cell [Escherichia coli (E. coli)] containing droplets by their size differences. Pinched-flow separation method is integrated to sort out droplets of certain sizes which have high probability of containing one cell. Consequently, we were able to obtain more than 50% of droplets having single E. coli inside, keeping the proportion of multiple-cell containing droplets less than 16%.-
dc.publisherAmer Inst Phys-
dc.titleRandom breakup of microdroplets for single-cell encapsulation-
dc.title.alternativeRandom breakup of microdroplets for single-cell encapsulation-
dc.typeArticle-
dc.citation.titleApplied Physics Letters-
dc.citation.number15-
dc.citation.endPage153703-
dc.citation.startPage153703-
dc.citation.volume97-
dc.contributor.affiliatedAuthorSeung Goo Lee-
dc.contributor.alternativeName엄유진-
dc.contributor.alternativeName이승구-
dc.contributor.alternativeName박제균-
dc.identifier.bibliographicCitationApplied Physics Letters, vol. 97, no. 15, pp. 153703-153703-
dc.identifier.doi10.1063/1.3500980-
dc.description.journalClassY-
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
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