DC Field | Value | Language |
---|---|---|
dc.contributor.author | E Um | - |
dc.contributor.author | Seung Goo Lee | - |
dc.contributor.author | J K Park | - |
dc.date.accessioned | 2017-04-19T09:19:51Z | - |
dc.date.available | 2017-04-19T09:19:51Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0003-6951 | - |
dc.identifier.uri | 10.1063/1.3500980 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/9761 | - |
dc.description.abstract | Microfluidic droplet-based technology enables encapsulation of cells in the isolated aqueous chambers surrounded by immiscible fluid but single-cell encapsulation efficiency is usually less than 30%. In this letter, we introduce a simple microgroove structure to break droplets into random sizes which further allows collecting of single-cell [Escherichia coli (E. coli)] containing droplets by their size differences. Pinched-flow separation method is integrated to sort out droplets of certain sizes which have high probability of containing one cell. Consequently, we were able to obtain more than 50% of droplets having single E. coli inside, keeping the proportion of multiple-cell containing droplets less than 16%. | - |
dc.publisher | Amer Inst Phys | - |
dc.title | Random breakup of microdroplets for single-cell encapsulation | - |
dc.title.alternative | Random breakup of microdroplets for single-cell encapsulation | - |
dc.type | Article | - |
dc.citation.title | Applied Physics Letters | - |
dc.citation.number | 15 | - |
dc.citation.endPage | 153703 | - |
dc.citation.startPage | 153703 | - |
dc.citation.volume | 97 | - |
dc.contributor.affiliatedAuthor | Seung Goo Lee | - |
dc.contributor.alternativeName | 엄유진 | - |
dc.contributor.alternativeName | 이승구 | - |
dc.contributor.alternativeName | 박제균 | - |
dc.identifier.bibliographicCitation | Applied Physics Letters, vol. 97, no. 15, pp. 153703-153703 | - |
dc.identifier.doi | 10.1063/1.3500980 | - |
dc.description.journalClass | Y | - |
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