Proteome analysis of arabidopsis seedlings exposed to bacterial volatiles

Cited 91 time in scopus
Metadata Downloads
Title
Proteome analysis of arabidopsis seedlings exposed to bacterial volatiles
Author(s)
Y S Kwon; Choong-Min RyuSoo-Hyun Lee; Hyo Bee Park; K S Han; J H Lee; K Lee; W S Chung; M J Jeong; H K Kim; D W Bae
Bibliographic Citation
Planta, vol. 232, no. 6, pp. 1355-1370
Publication Year
2010
Abstract
Plant root-associated bacteria (rhizobacteria) elicit plant basal immunity referred to as induced systemic resistance (ISR) against multiple pathogens. Among multi-bacterial determinants involving such ISR, the induction of ISR and promotion of growth by bacterial volatile compounds was previously reported. To exploit global de novo expression of plant proteins by bacterial volatiles, proteomic analysis was performed after exposure of Arabidopsis plants to the rhizobacterium Bacillus subtilis GB03. Ethylene biosynthesis enzymes were significantly up-regulated. Analysis by quantitative reverse transcriptase polymerase chain reaction confirmed that ethylene biosynthesis-related genes SAM-2, ACS4, ACS12, and ACO2 as well as ethylene response genes, ERF1, GST2, and CHIB were up-regulated by the exposure to bacterial volatiles. More interestingly, the emission of bacterial volatiles significantly up-regulated both key defense mechanisms mediated by jasmonic acid and salicylic acid signaling pathways. In addition, high accumulation of antioxidant proteins also provided evidence of decreased sensitivity to reactive oxygen species during the elicitation of ISR by bacterial volatiles. The present results suggest that the proteomic analysis of plant defense responses in bacterial volatile-mediated ISR can reveal the mechanisms of plant basal defenses orchestrated by endogenous ethylene production pathways and the generation of reactive oxygen species.
Keyword
Bacterial volatilesEthyleneInduced systemic resistancePlant growth-promoting rhizobacteriaProteomics
ISSN
0032-0935
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00425-010-1259-x
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.