Evaluation of NPTII gene transfer from transgenic chili pepper to culturable microorganisms in rhizosphere soil = 형질전환 고추로부터 근권 토양 미생물로의 수평유전자이동 조사

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dc.contributor.authorB Lee-
dc.contributor.authorJ Y Park-
dc.contributor.authorKee Woong Park-
dc.contributor.authorC H Harn-
dc.contributor.authorHwan Mook Kim-
dc.contributor.authorChang-Gi Kim-
dc.date.accessioned2017-04-19T09:19:53Z-
dc.date.available2017-04-19T09:19:53Z-
dc.date.issued2010-
dc.identifier.issn1225-8504-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9779-
dc.description.abstractThe possible gene transfer from transgenic chili pepper to soil bacteria in rhizosphere was evaluated. DNA was extracted from rhizosphere soil collected from growing transgenic chili pepper (Capsicum annuum L.) resistant to cucumber mosaic virus (CMV) and control line, and PCR analysis was performed with specific primer for transgene in the transgenic chili pepper. The results of the PCR targeting NPTII gene indicated that the transgene was present in the rhizosphere soil samples in all collecting times. To investigate the cause of the transgene detection in the samples, we isolated microorganisms from the soil and cultured them on bacterial and fungal medium containing kanamycin. About 430 thousands of bacterial colonies and 160 thousands of fungal colonies were investigated, however, colonies resistant to kanamycin were not detected, indicating gene transfer from transgenic chili pepper to culturable rhizosphere soil microorganisms was not occurred in the present study.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleEvaluation of NPTII gene transfer from transgenic chili pepper to culturable microorganisms in rhizosphere soil = 형질전환 고추로부터 근권 토양 미생물로의 수평유전자이동 조사-
dc.title.alternativeEvaluation of NPTII gene transfer from transgenic chili pepper to culturable microorganisms in rhizosphere soil-
dc.typeArticle-
dc.citation.titleKorean Journal of International Agriculture-
dc.citation.number3-
dc.citation.endPage292-
dc.citation.startPage288-
dc.citation.volume22-
dc.contributor.affiliatedAuthorKee Woong Park-
dc.contributor.affiliatedAuthorHwan Mook Kim-
dc.contributor.affiliatedAuthorChang-Gi Kim-
dc.contributor.alternativeName이범규-
dc.contributor.alternativeName박지영-
dc.contributor.alternativeName박기웅-
dc.contributor.alternativeName한지학-
dc.contributor.alternativeName김환묵-
dc.contributor.alternativeName김창기-
dc.identifier.bibliographicCitationKorean Journal of International Agriculture, vol. 22, no. 3, pp. 288-292-
dc.subject.keywordCapsicum annuum-
dc.subject.keywordchili pepper-
dc.subject.keywordgene transfer-
dc.subject.keywordmicroorganisms-
dc.subject.keywordrhizosphere-
dc.subject.keywordtransgenic crop-
dc.subject.localCapsicum annum-
dc.subject.localCapsicum annuum-
dc.subject.localCapsicum annuum L.-
dc.subject.localcapsicum annuum-
dc.subject.localChili pepper-
dc.subject.localChili pepper (Capsicum annum)-
dc.subject.localChili pepper (Capsicum annuum)-
dc.subject.localchili pepper-
dc.subject.localgene transfer-
dc.subject.localmicroorganism-
dc.subject.localmicroorganisms-
dc.subject.localMicroorganism-
dc.subject.localMicroorganisms-
dc.subject.localmicroorganims-
dc.subject.localRhizosphere-
dc.subject.localrhizosphere-
dc.subject.localTransgenic crop-
dc.subject.localtransgenic crop-
dc.description.journalClassN-
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Division of Bio Technology Innovation > Bio-Evaluation Center > 1. Journal Articles
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