Proteomic analysis of oxidative stress-induced neuronal cell death by using two-dimensional fluorescence difference gel electrophoresi

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Title
Proteomic analysis of oxidative stress-induced neuronal cell death by using two-dimensional fluorescence difference gel electrophoresi
Author(s)
Eun Young Kim; Tae-Sung Yoon; Young Jae Bahn; Dae Gwin Jeong; M R Park; Sang Jeon Chung; Sung Goo ParkByoung Chul ParkSang Chul Lee; S E Ryu; Kwang-Hee Bae
Bibliographic Citation
International Journal of Molecular Medicine, vol. 26, no. 6, pp. 829-835
Publication Year
2010
Abstract
Oxidative stress has been implicated in a number of neurological disorders, including cerebral ischemia and neuro-degenerative diseases. Comprehensive proteomic studies were carried out using an immortalized mouse hippocampal cell line, HT22, exhibiting oxidative stress-mediated cell death upon glutamate treatment. Two-dimensional fluorescence difference gel electrophoresis (2D DIGE) of subcellular organelle fractions revealed that significant numbers of proteins showed quantitative changes during HT22 cell death, among which a total of 51 proteins were identified by mass spectrometry. The identified proteins indicate that HT22 cell death occurs through perturbations in mitochondrial function, changes in translational elongation machinery, and translocation of proteins across subcellular organelles. This list of proteins may shed light on oxidative stress-mediated neuronal cell death.
Keyword
HT22Oxidative stressProteomicsReactive oxygen speciesTwo-dimensional fluorescence difference gel electrophoresis
ISSN
1107-3756
Publisher
Spandidos Publ Ltd
DOI
http://dx.doi.org/10.3892/ijmm-00000531
Type
Article
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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