Identification of tyrosine-nitrated proteins in HT22 hippocampal cells during glutamate-induced oxidative stress

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Title
Identification of tyrosine-nitrated proteins in HT22 hippocampal cells during glutamate-induced oxidative stress
Author(s)
Sun Woo Yoon; S Kang; S E Ryu; Haryoung Poo
Bibliographic Citation
Cell Proliferation, vol. 43, no. 6, pp. 584-593
Publication Year
2010
Abstract
Objectives: Nitration of tyrosine residues in protein is a post-translational modification, which occurs under oxidative stress, and is associated with several neurodegenerative diseases. To understand the role of nitrated proteins in oxidative stress-induced cell death, we identified nitrated proteins and checked correlation of their nitration in glutamate-induced HT22 cell death.Materials and methods: Nitrated proteins were detected by western blotting using an anti-nitrotyrosine antibody, extracted from matching reference 2-dimensional electrophoresis gels, and identified with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.Results: Glutamate treatment induced apoptosis in HT22 cells, while reactive oxygen species (ROS) inhibitor or neuronal nitric oxide synthase (nNOS) inhibitor blocked glutamate-induced HT22 cell death. Nitration levels of 13 proteins were increased after glutamate stimulation; six of them were involved in regulation of energy production and two were related to apoptosis. The other nitrated proteins were associated with calcium signal modulation, ER dysfunction, or were of unknown function.Conclusions: The 13 tyrosine-nitrated proteins were detected in these glutamate-treated HT22 cells. Results demonstrated that cell death, ROS accumulation and nNOS expression were related to nitration of protein tyrosine in the glutamate-stimulated cells.
ISSN
0960-7722
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/j.1365-2184.2010.00708.x
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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