A comparative study of in- and post-source decays of peptide and preformed ions in matrix-assisted laser desorption ionization time-of-flight mass spectrometry: Effective temperature and matrix effect

Cited 28 time in scopus
Metadata Downloads
Title
A comparative study of in- and post-source decays of peptide and preformed ions in matrix-assisted laser desorption ionization time-of-flight mass spectrometry: Effective temperature and matrix effect
Author(s)
S H Yoon; Jeong Hee Moon; M S Kim
Bibliographic Citation
Journal of American Society for Mass Spectrometry, vol. 21, no. 11, pp. 1876-1883
Publication Year
2010
Abstract
In-source decay (ISD) and post-source decay (PSD) of a peptide ion ([Y6 + H]+) and a preformed ion (benzyltriphenylphosphonium, BTPP) generated by matrix-assisted laser desorption ionization (MALDI) were investigated with time-of-flight mass spectrometry. α-Cyano-4-hydroxycinammic acid (CHCA) and 2,5-dihydroxybenzoic acid (DHB) were used as matrices. For both ions, ISD yield was unaffected by delay time, indicating rapid termination of ISD. This was taken as evidence for rapid expansion cooling of hot "early" plume formed in MALDI. CHCA was hotter than DHB for [Y6 + H]+ while the matrix effect was insignificant for BTPP. The "early" plume temperature estimated utilizing previous kinetic results was 800-900 K, versus 400-500 K for "late" plume. The results support our previous finding that the temperature of peptide ions interrogated by tandem mass spectrometry was lower than most rough estimates of MALDI temperature.
ISSN
1044-0305
Publisher
Amer Chem Soc
DOI
http://dx.doi.org/10.1016/j.jasms.2010.07.001
Type
Article
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.