Open Access@KRIBBSynthetic Biology and Bioengineering Research InstituteCell Factory Research Center1. Journal Articles
Complete reductive dechlorination of tetrachloroethene to ethene by anaerobic microbial enrichment culture developed from sediment
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- Title
- Complete reductive dechlorination of tetrachloroethene to ethene by anaerobic microbial enrichment culture developed from sediment
- Author(s)
- Byung-Hyuk Kim; K H Baek; Dae-Hyun Cho; Y Sung; S C Koh; Chi-Yong Ahn; Hee-Mock Oh; Hee-Sik Kim
- Bibliographic Citation
- Biotechnology Letters, vol. 32, no. 12, pp. 1829-1835
- Publication Year
- 2010
- Abstract
- A mixed, anaerobic microbial enrichment culture, AMEC-4P, was developed that uses lactate as the electron donor for the reductive dechlorination of tetrachloroethene (PCE) to ethene. AMEC-4P consistently and completely converted 2 mM PCE to cis-1,2-dichloroethene (cis-DCE) within 13 days, and the intermediate, cis-DCE, was then completely dechlorinated to ethene after 130 days. Dechlorination rates for PCE to cis-DCE, cis-DCE to VC, and VC to ethene were 243, 27, and 41 μmol/l/day, respectively. Geobacter lovleyi and a Dehalococcoides sp. were identified from their 16S rRNA sequences to be the dominant phylotypes in AMEC-4P.
- Keyword
- Anaerobic microbial enrichment cultureDechlorinationMicrobial community analysisTetrachloroethene
- ISSN
- 0141-5492
- Publisher
- Springer
- Type
- Article
- Appears in Collections:
- Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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