The DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis

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Title
The DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis
Author(s)
H S Kim; S J Kim; N Abbasi; R A Bressan; D J Yun; S D Yoo; Suk Yoon Kwon; S B Choi
Bibliographic Citation
Plant Journal, vol. 64, no. 3, pp. 524-535
Publication Year
2010
Abstract
Dof proteins are transcription factors that have a conserved single zinc finger DNA-binding domain. In this study, we isolated an activation tagging mutant Dof5.1-D exhibiting an upward-curling leaf phenotype due to enhanced expression of the REV gene that is required for establishing adaxialabaxial polarity. Dof5.1-D plants also had reduced transcript levels for IAA6 and IAA19 genes, indicating an altered auxin biosynthesis in Dof5.1-D. An electrophoretic mobility shift assay using the Dof5.1 DNA-binding motif and the REV promoter region indicated that the DNA-binding domain of Dof5.1 binds to a TAAAGT motif located in the 5′-distal promoter region of the REV promoter. Further, transient and chromatin immunoprecipitation assays verified binding activity of the Dof5.1 DNA-binding motif with the REV promoter. Consistent with binding assays, constitutive over-expression of the Dof5.1 DNA-binding domain in wild-type plants caused a downward-curling phenotype, whereas crossing Dof5.1-D to a rev mutant reverted the upward-curling phenotype of the Dof5.1-D mutant leaf to the wild-type. These results suggest that the Dof5.1 protein directly binds to the REV promoter and thereby regulates adaxialabaxial polarity.
Keyword
Activation tagging mutantDof transcription factorDof5.1Leaf polarityRevoluta
ISSN
0960-7412
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/j.1365-313X.2010.04346.x
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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