Expression profiling of calcium induced genes in cultured human keratinocytes

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dc.contributor.authorJ S Lee-
dc.contributor.authorMirang Kim-
dc.contributor.authorNam-Soon Kim-
dc.contributor.authorYong Sung Kim-
dc.contributor.authorJ M Yang-
dc.contributor.authorA Y Cho-
dc.contributor.authorY Lee-
dc.contributor.authorC D Kim-
dc.contributor.authorJ H Lee-
dc.date.accessioned2017-04-19T09:20:17Z-
dc.date.available2017-04-19T09:20:17Z-
dc.date.issued2010-
dc.identifier.issn1011-8934-
dc.identifier.uri10.3346/jkms.2010.25.4.619ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9834-
dc.description.abstractTerminal differentiation of skin keratinocytes is a vertically directed multi-step process that is tightly controlled by the sequential expression of a variety of genes. To examine the gene expression profile in calcium-induced keratinocyte differentiation, we constructed a normalized cDNA library using mRNA isolated from these calcium-treated keratinocytes. After sequencing about 10,000 clones, we were able to obtain 4,104 independent genes. They consisted of 3,699 annotated genes and 405 expressed sequence tags (ESTs). Some were the genes involved in constituting epidermal structures and others were unknown genes that are probably associated with keratinocytes. In particular, we were able to identify genes located at the chromosome 1q21, the locus for the epidermal differentiation complex, and 19q13.1, another probable locus for epidermal differentiation-related gene clusters. One EST located at the chromosome 19q13.1 showed increased expression by calcium treatment, suggesting a novel candidate gene relevant to keratinocyte differentiation. These results demonstrate the complexity of the transcriptional profile of keratinocytes, providing important clues on which to base further investigations of the molecular events underlying keratinocyte differentiation.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleExpression profiling of calcium induced genes in cultured human keratinocytes-
dc.title.alternativeExpression profiling of calcium induced genes in cultured human keratinocytes-
dc.typeArticle-
dc.citation.titleJournal of Korean Medical Science-
dc.citation.number4-
dc.citation.endPage625-
dc.citation.startPage619-
dc.citation.volume25-
dc.contributor.affiliatedAuthorMirang Kim-
dc.contributor.affiliatedAuthorNam-Soon Kim-
dc.contributor.affiliatedAuthorYong Sung Kim-
dc.contributor.alternativeName이정석-
dc.contributor.alternativeName김미랑-
dc.contributor.alternativeName김남순-
dc.contributor.alternativeName김용성-
dc.contributor.alternativeName양준모-
dc.contributor.alternativeName조아영-
dc.contributor.alternativeName이영-
dc.contributor.alternativeName김창덕-
dc.contributor.alternativeName이정훈-
dc.identifier.bibliographicCitationJournal of Korean Medical Science, vol. 25, no. 4, pp. 619-625-
dc.identifier.doi10.3346/jkms.2010.25.4.619-
dc.subject.keywordCalcium-
dc.subject.keywordGene expression profiling-
dc.subject.keywordKeratinocyte differentiation-
dc.subject.keywordNormalized library-
dc.subject.localcalcium-
dc.subject.localCalcium-
dc.subject.localGene expression profiling.-
dc.subject.localGene expression profiling-
dc.subject.localgene expression profiling-
dc.subject.localKeratinocyte differentiation-
dc.subject.localNormalized library-
dc.description.journalClassY-
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
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