Implication of mouse Vps26b-Vps29-Vps35 retromer complex in sortilin trafficking = 솔트린 수송에 관여하는 리트로머 분자

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Title
Implication of mouse Vps26b-Vps29-Vps35 retromer complex in sortilin trafficking = 솔트린 수송에 관여하는 리트로머 분자
Author(s)
Ekyune Kim; Youngjeon Lee; H J Lee; Ji Su KimBong Seok SongJae Won Huh; Sang Rae Lee; Sun-Uk Kim; Kim Sang-Hyun; Y Hong; I Shim; Kyu Tae Chang
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 403, no. 2, pp. 167-171
Publication Year
2010
Abstract
The retromer complex, which mediates retrograde transport from endosomes to the trans-Golgi network, is a heteropentameric complex that contains a multifunctional cargo recognition heterotrimer consisted of the vacuolar protein sorting (Vps) subunits Vps26, Vps29, and Vps35. In mammals, there are two different isoforms of Vps26, Vps26a and Vps26b, that localize to the endosome, and to the plasma membrane, respectively. To elucidate the biological significance of the Vps26b isoform, we generated Vps26b knockout mice and studied their molecular, histological, and behavioral phenotypes. We found that the loss of Vps26b results in no significant defects in the behavior, body size, and health of the mice. Vps26b-deficient mice showed a severe reduction of Vps35 protein at cellular level and lacked the Vps26b-Vps29-Vps35 retromer complex, despite the normal presence of the Vps26a-Vps29-Vps35 retromer complex. Relatively, the amount of sortilin was increased approximately 20% in the Vps26b-deficient mice, whereas the sorLA was normal. These results suggest that mouse Vps26b-Vps29-Vps35 retromer complex is implicated in the transport of sortilin from endosomes to the trans-Golgi network (TGN).
Keyword
EndosomesRetromer complexTrans-Golgi network (TGN)Vacuolar protein sorting 26b (Vps26b)Vps26b-deficient mice
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bbrc.2010.10.121
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > National Primate Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
Ochang Branch Institute > Division of National Bio-Infrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
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