Kinetics of Zn(2+)-induced brain type creatine kinase unfolding and aggregation

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dc.contributor.authorH Mu-
dc.contributor.authorZ R Lu-
dc.contributor.authorD Park-
dc.contributor.authorB C Kim-
dc.contributor.authorJong Hwa Park-
dc.contributor.authorF Zou-
dc.contributor.authorJ M Yang-
dc.contributor.authorS Li-
dc.contributor.authorY D Park-
dc.contributor.authorH C Zou-
dc.contributor.authorH M Zhou-
dc.date.accessioned2017-04-19T09:20:51Z-
dc.date.available2017-04-19T09:20:51Z-
dc.date.issued2010-
dc.identifier.issn0273-2289-
dc.identifier.uri10.1007/s12010-009-8574-3ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9874-
dc.description.abstractWe studied the effect of Zn2+ on the folding and aggregation of brain creatine kinase (CK-BB). We developed a method to purify CK-BB from rabbit brain and conducted inhibition kinetics and unfolding studies of CK-BB. Zn 2+ conspicuously aggregated and osmolytes, such as glycine and proline, were able to suppress the formation of aggregates and protect the enzymatic activity against Zn2+. These results suggest that Zn 2+ might act as a risk factor for CK-BB in the brain under certain conditions, and some osmolytes may help CK-BB to sustain the active state when Zn2+ is present. Our study provides useful information regarding the effect of Zn2+ on brain-derived metabolic enzymes, especially those that are putatively related to brain disease. Furthermore, our study suggests that although Zn2+ may induce CK-BB inactivation and misfolding, the ability of some abundant proteins and osmolytes to chelate Zn2+ nonspecifically may protect CK-BB and allow it to exist in the active form.-
dc.publisherSpringer-
dc.titleKinetics of Zn(2+)-induced brain type creatine kinase unfolding and aggregation-
dc.title.alternativeKinetics of Zn(2+)-induced brain type creatine kinase unfolding and aggregation-
dc.typeArticle-
dc.citation.titleApplied Biochemistry and Biotechnology-
dc.citation.number5-
dc.citation.endPage1320-
dc.citation.startPage1309-
dc.citation.volume160-
dc.contributor.affiliatedAuthorJong Hwa Park-
dc.contributor.alternativeNameMu-
dc.contributor.alternativeNameLu-
dc.contributor.alternativeName박대의-
dc.contributor.alternativeName김병철-
dc.contributor.alternativeName박종화-
dc.contributor.alternativeNameZou-
dc.contributor.alternativeName양준모-
dc.contributor.alternativeNameLi-
dc.contributor.alternativeName박용두-
dc.contributor.alternativeNameZou-
dc.contributor.alternativeNameZhou-
dc.identifier.bibliographicCitationApplied Biochemistry and Biotechnology, vol. 160, no. 5, pp. 1309-1320-
dc.identifier.doi10.1007/s12010-009-8574-3-
dc.subject.keywordAggregation-
dc.subject.keywordBrain-type-
dc.subject.keywordCreatine kinase-
dc.subject.keywordFolding-
dc.subject.keywordZn2+-
dc.subject.localAggregation-
dc.subject.localaggregation-
dc.subject.localBrain-type-
dc.subject.localCreatine kinase-
dc.subject.localcreatine kinase-
dc.subject.localFolding-
dc.subject.localZn2+-
dc.description.journalClassY-
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