DC Field | Value | Language |
---|---|---|
dc.contributor.author | S Lee | - |
dc.contributor.author | S Y Kim | - |
dc.contributor.author | Kyoung Sook Park | - |
dc.contributor.author | Bong Hyun Chung | - |
dc.contributor.author | S W Kim | - |
dc.date.accessioned | 2017-04-19T09:20:52Z | - |
dc.date.available | 2017-04-19T09:20:52Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0022-2313 | - |
dc.identifier.uri | 10.1016/j.jlumin.2010.10.012 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/9881 | - |
dc.description.abstract | This paper studies the conformational change of the binding protein by a fluorescence lifetime method. As a model protein, maltose binding protein (MBP) where enhanced cyan protein (ECFP) and enhanced yellow fluorescent protein (EYFP) were genetically fused to act as a donor and an acceptor in Frster resonance energy transfer (FRET) was used. The ECFP and the EYFP were linked to the C-terminal and N-terminal regions of the MBP, respectively. In order to investigate the conformational change of the MBP, the lifetime of the ECFP, which acts as a donor in the ECFP:MBP:EYFP fusion protein, was analyzed during the FRET process. We observed that two lifetime components exist when the ECFP is linked to the MBP and that the lifetime of the ECFP is shortened when ECFP:MBP:EYFP protein undergoes a conformational change as a result of the maltose binding. In addition, we observed that the lifetime of the donor is gradually shorter in the ECFP:MBP:EYFP fusion protein as the maltose concentration increases. By a lifetime analysis and simulation study, we found that the participant rate of the ECFP:MBP:EYFP protein in FRET is the main cause of the donor lifetime shortening in relation to the increase of the maltose concentration. | - |
dc.publisher | Elsevier | - |
dc.title | Fluorescence lifetime study of ECFP/EYFP labeled MBP in Frster resonance energy transfer | - |
dc.title.alternative | Fluorescence lifetime study of ECFP/EYFP labeled MBP in Frster resonance energy transfer | - |
dc.type | Article | - |
dc.citation.title | Journal of Luminescence | - |
dc.citation.number | 2 | - |
dc.citation.endPage | 279 | - |
dc.citation.startPage | 275 | - |
dc.citation.volume | 131 | - |
dc.contributor.affiliatedAuthor | Kyoung Sook Park | - |
dc.contributor.affiliatedAuthor | Bong Hyun Chung | - |
dc.contributor.alternativeName | 이순혁 | - |
dc.contributor.alternativeName | 김수용 | - |
dc.contributor.alternativeName | 박경숙 | - |
dc.contributor.alternativeName | 정봉현 | - |
dc.contributor.alternativeName | 김석원 | - |
dc.identifier.bibliographicCitation | Journal of Luminescence, vol. 131, no. 2, pp. 275-279 | - |
dc.identifier.doi | 10.1016/j.jlumin.2010.10.012 | - |
dc.subject.keyword | FRET | - |
dc.subject.keyword | Lifetime | - |
dc.subject.keyword | Maltose binding protein | - |
dc.subject.keyword | TCSPC | - |
dc.subject.local | Fluorescence resonance energy transfer, FRET | - |
dc.subject.local | fluorescence resonance energy transfer | - |
dc.subject.local | FRET | - |
dc.subject.local | Fluorescence resonance energy transfer | - |
dc.subject.local | Fluorescence resonance energy transfer (FRET) | - |
dc.subject.local | Lifetime | - |
dc.subject.local | Maltose Binding Protein | - |
dc.subject.local | maltose binding protein | - |
dc.subject.local | maltose binding protein (MBP) | - |
dc.subject.local | Maltose-binding protein | - |
dc.subject.local | Maltose binding protein, MBP | - |
dc.subject.local | Maltose binding protein | - |
dc.subject.local | TCSPC | - |
dc.description.journalClass | Y | - |
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