In vivo effects of NbSiR silencing on chloroplast development in Nicotiana benthamiana

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dc.contributor.authorY W Kang-
dc.contributor.authorJ Y Lee-
dc.contributor.authorY Jeon-
dc.contributor.authorG W Cheong-
dc.contributor.authorMoonil Kim-
dc.contributor.authorH S Pai-
dc.date.accessioned2017-04-19T09:20:54Z-
dc.date.available2017-04-19T09:20:54Z-
dc.date.issued2010-
dc.identifier.issn0167-4412-
dc.identifier.uri10.1007/s11103-009-9593-8ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9887-
dc.description.abstractSulfite reductase (SiR) performs dual functions, acting as a sulfur assimilation enzyme and as a chloroplast (cp-) nucleoid binding protein. In this study, we examined the in vivo effects of SiR deficiency on chloroplast development in Nicotiana benthamiana. Virus-induced gene silencing of NbSiR resulted in leaf yellowing and growth retardation phenotypes, which were not rescued by cysteine supplementation. NbSiR:GFP fusion protein was targeted to chloroplasts and colocalized with cp-nucleoids. Recombinant full-length NbSiR protein and the C-terminal half of NbSiR possessed cp-DNA compaction activities in vitro, and expression of full-length NbSiR in E. coli caused condensation of genomic DNA. NbSiR silencing differentially affected expression of plastid-encoded genes, inhibiting expression of several genes more severely than others. In the later stages, depletion of NbSiR resulted in chloroplast ablation. In NbSiR-silenced plants, enlarged cp-nucleoids containing an increased amount of cp-DNA were observed in the middle of the abnormal chloroplasts, and the cp-DNAs were predominantly of subgenomic sizes based on pulse field gel electrophoresis. The abnormal chloroplasts developed prolamellar body-like cubic lipid structures in the light without accumulating NADPH:protochlorophyllide oxidoreductase proteins. Our results suggest that NbSiR plays a role in cp-nucleoid metabolism, plastid gene expression, and thylakoid membrane development.-
dc.publisherSpringer-
dc.titleIn vivo effects of NbSiR silencing on chloroplast development in Nicotiana benthamiana-
dc.title.alternativeIn vivo effects of NbSiR silencing on chloroplast development in Nicotiana benthamiana-
dc.typeArticle-
dc.citation.titlePlant Molecular Biology-
dc.citation.number6-
dc.citation.endPage583-
dc.citation.startPage569-
dc.citation.volume72-
dc.contributor.affiliatedAuthorMoonil Kim-
dc.contributor.alternativeName강용원-
dc.contributor.alternativeName이재용-
dc.contributor.alternativeName전영-
dc.contributor.alternativeName정광원-
dc.contributor.alternativeName김문일-
dc.contributor.alternativeName배현숙-
dc.identifier.bibliographicCitationPlant Molecular Biology, vol. 72, no. 6, pp. 569-583-
dc.identifier.doi10.1007/s11103-009-9593-8-
dc.subject.keywordChloroplast nucleoids-
dc.subject.keywordDNA condensation-
dc.subject.keywordPlastid transcription-
dc.subject.keywordProlamellar body-
dc.subject.keywordPulse field gel electrophoresis-
dc.subject.keywordVirus-induced gene silencing-
dc.subject.localChloroplast nucleoids-
dc.subject.localDNA condensation-
dc.subject.localPlastid transcription-
dc.subject.localProlamellar body-
dc.subject.localPulse field gel electrophoresis-
dc.subject.localpulse-field gel electrophoresis (PFGE)-
dc.subject.localpulsed-field gel electrophoresis-
dc.subject.localpulsed-fielded gel electrophoresis-
dc.subject.localvirus-induced gene silencing-
dc.subject.localVIGS-
dc.subject.localVirus-induced gene silencing-
dc.subject.localVirus-induced gene silencing (VIGS)-
dc.description.journalClassY-
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Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
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