DC Field | Value | Language |
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dc.contributor.author | Y W Kang | - |
dc.contributor.author | J Y Lee | - |
dc.contributor.author | Y Jeon | - |
dc.contributor.author | G W Cheong | - |
dc.contributor.author | Moonil Kim | - |
dc.contributor.author | H S Pai | - |
dc.date.accessioned | 2017-04-19T09:20:54Z | - |
dc.date.available | 2017-04-19T09:20:54Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0167-4412 | - |
dc.identifier.uri | 10.1007/s11103-009-9593-8 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/9887 | - |
dc.description.abstract | Sulfite reductase (SiR) performs dual functions, acting as a sulfur assimilation enzyme and as a chloroplast (cp-) nucleoid binding protein. In this study, we examined the in vivo effects of SiR deficiency on chloroplast development in Nicotiana benthamiana. Virus-induced gene silencing of NbSiR resulted in leaf yellowing and growth retardation phenotypes, which were not rescued by cysteine supplementation. NbSiR:GFP fusion protein was targeted to chloroplasts and colocalized with cp-nucleoids. Recombinant full-length NbSiR protein and the C-terminal half of NbSiR possessed cp-DNA compaction activities in vitro, and expression of full-length NbSiR in E. coli caused condensation of genomic DNA. NbSiR silencing differentially affected expression of plastid-encoded genes, inhibiting expression of several genes more severely than others. In the later stages, depletion of NbSiR resulted in chloroplast ablation. In NbSiR-silenced plants, enlarged cp-nucleoids containing an increased amount of cp-DNA were observed in the middle of the abnormal chloroplasts, and the cp-DNAs were predominantly of subgenomic sizes based on pulse field gel electrophoresis. The abnormal chloroplasts developed prolamellar body-like cubic lipid structures in the light without accumulating NADPH:protochlorophyllide oxidoreductase proteins. Our results suggest that NbSiR plays a role in cp-nucleoid metabolism, plastid gene expression, and thylakoid membrane development. | - |
dc.publisher | Springer | - |
dc.title | In vivo effects of NbSiR silencing on chloroplast development in Nicotiana benthamiana | - |
dc.title.alternative | In vivo effects of NbSiR silencing on chloroplast development in Nicotiana benthamiana | - |
dc.type | Article | - |
dc.citation.title | Plant Molecular Biology | - |
dc.citation.number | 6 | - |
dc.citation.endPage | 583 | - |
dc.citation.startPage | 569 | - |
dc.citation.volume | 72 | - |
dc.contributor.affiliatedAuthor | Moonil Kim | - |
dc.contributor.alternativeName | 강용원 | - |
dc.contributor.alternativeName | 이재용 | - |
dc.contributor.alternativeName | 전영 | - |
dc.contributor.alternativeName | 정광원 | - |
dc.contributor.alternativeName | 김문일 | - |
dc.contributor.alternativeName | 배현숙 | - |
dc.identifier.bibliographicCitation | Plant Molecular Biology, vol. 72, no. 6, pp. 569-583 | - |
dc.identifier.doi | 10.1007/s11103-009-9593-8 | - |
dc.subject.keyword | Chloroplast nucleoids | - |
dc.subject.keyword | DNA condensation | - |
dc.subject.keyword | Plastid transcription | - |
dc.subject.keyword | Prolamellar body | - |
dc.subject.keyword | Pulse field gel electrophoresis | - |
dc.subject.keyword | Virus-induced gene silencing | - |
dc.subject.local | Chloroplast nucleoids | - |
dc.subject.local | DNA condensation | - |
dc.subject.local | Plastid transcription | - |
dc.subject.local | Prolamellar body | - |
dc.subject.local | Pulse field gel electrophoresis | - |
dc.subject.local | pulse-field gel electrophoresis (PFGE) | - |
dc.subject.local | pulsed-field gel electrophoresis | - |
dc.subject.local | pulsed-fielded gel electrophoresis | - |
dc.subject.local | virus-induced gene silencing | - |
dc.subject.local | VIGS | - |
dc.subject.local | Virus-induced gene silencing | - |
dc.subject.local | Virus-induced gene silencing (VIGS) | - |
dc.description.journalClass | Y | - |
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