Qualitative and quantitative detection of agricultural microorganisms expressing iturin and mop cyclase in soils

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dc.contributor.authorSung Eun Kim-
dc.contributor.authorJae Sun Moon-
dc.contributor.authorWon Sik Choi-
dc.contributor.authorE N Lee-
dc.contributor.authorS H Lee-
dc.contributor.authorSung Uk Kim-
dc.date.accessioned2017-04-19T09:21:01Z-
dc.date.available2017-04-19T09:21:01Z-
dc.date.issued2010-
dc.identifier.issn0021-8561-
dc.identifier.uri10.1021/jf103216gko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9916-
dc.description.abstractThe environmental release of genetically engineered microorganisms (GEMs) to improve agriculture or remediate environmental hazards has raised concern over the fate of the organisms and their engineered genes. To detect the microorganisms released into the environment at the molecular level, Bacillus subtilis KB producing iturin and Pseudomonas fluorescens MX1 carrying the moc (mannityl opine catabolism) region from the Agrobacterium tumefaciens were employed as model microorganisms. Using specific fusion primers and the TaqMan probes, qualitative and quantitative detections of the model organisms by PCR and real-time PCR were conducted employing a small-scale soil-core device and pots during the six month period. The data indicate that the model bacteria can be easily detected by qualitative and quantitative methods in the test systems employed, and they do not give significant impacts on the other bacteria in soils on the Southern blotting analysis, although long-term observation may be needed. ⓒ 2010 American Chemical Society.-
dc.publisherAmer Chem Soc-
dc.titleQualitative and quantitative detection of agricultural microorganisms expressing iturin and mop cyclase in soils-
dc.title.alternativeQualitative and quantitative detection of agricultural microorganisms expressing iturin and mop cyclase in soils-
dc.typeArticle-
dc.citation.titleJournal of Agricultural and Food Chemistry-
dc.citation.number24-
dc.citation.endPage12663-
dc.citation.startPage12657-
dc.citation.volume58-
dc.contributor.affiliatedAuthorSung Eun Kim-
dc.contributor.affiliatedAuthorJae Sun Moon-
dc.contributor.affiliatedAuthorWon Sik Choi-
dc.contributor.affiliatedAuthorSung Uk Kim-
dc.contributor.alternativeName김성은-
dc.contributor.alternativeName문제선-
dc.contributor.alternativeName최원식-
dc.contributor.alternativeName이은나-
dc.contributor.alternativeName이상한-
dc.contributor.alternativeName김성욱-
dc.identifier.bibliographicCitationJournal of Agricultural and Food Chemistry, vol. 58, no. 24, pp. 12657-12663-
dc.identifier.doi10.1021/jf103216g-
dc.subject.keywordAgricultural microorganism-
dc.subject.keywordgenetically modified organism (GMO)-
dc.subject.keywordhorizontal gene transfer-
dc.subject.keyworditurin-
dc.subject.keywordmop cyclase-
dc.subject.keywordPGPR-
dc.subject.keywordreal-time PCR-
dc.subject.keywordsoil-core device-
dc.subject.localAgricultural microorganism-
dc.subject.localgenetically modified organism-
dc.subject.localgenetically modified organism (GMO)-
dc.subject.localGenetically modified organism-
dc.subject.localhorizontal gene transfer-
dc.subject.localHorizontal gene transfer-
dc.subject.localiturin-
dc.subject.localmop cyclase-
dc.subject.localplant growthpromoting rhizobacteria-
dc.subject.localPlant growth promoting rhizobacteria (PGPR)-
dc.subject.localPlant growthpromoting rhizobacteria (PGPR)-
dc.subject.localPlant growthpromoting rhizobacteria-
dc.subject.localPlant growth-promoting rhizobacteria-
dc.subject.localPlant growth-promoting rhizobacteria (PGPR)-
dc.subject.localPGPR-
dc.subject.localplant growth-promoting rhizobacteria (PGPR)-
dc.subject.localplant growth-promoting rhizobacteria-
dc.subject.localReal-time PCR-
dc.subject.localreal time PCR-
dc.subject.localReal time PCR-
dc.subject.localreal-time PCR-
dc.subject.localsoil-core device-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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