Epigenetic modification of retinoic acid-treated human embryonic stem cells

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Title
Epigenetic modification of retinoic acid-treated human embryonic stem cells
Author(s)
H S Cheong; H C Lee; B L Park; H Kim; M J Jang; Y M Han; Seon-Young Kim; Yong Sung Kim; H D Shin
Bibliographic Citation
BMB Reports, vol. 43, no. 12, pp. 830-835
Publication Year
2010
Abstract
Epigenetic modification of the genome through DNA methylation is the key to maintaining the differentiated state of human embryonic stem cells (hESCs), and it must be reset during differentiation by retinoic acid (RA) treatment. A genome-wide methylation/gene expression assay was performed in order to identify epigenetic modifications of RA-treated hESCs. Between undifferentiated and RA-treated hESCs, 166 differentially methylated CpG sites and 2,013 differentially expressed genes were discovered. Combined analysis of methylation and expression data revealed that 19 genes (STAP2, VAMP8, C10orf26, WFIKKN1, ELF3, C1QTNF6, C10orf10, MRGPRF, ARSE, LSAMP, CENTD3, LDB2, POU5F1, GSPT2, THY1, ZNF574, MSX1, SCMH1, and RARB) were highly correlated with each other. The results provided in this study will facilitate future investigations into the interplay between DNA methylation and gene expression through further functional and biological studies.
Keyword
DNA methylationEpigenetic modificationGene expressionHuman embryonic stem cellRetinoic acid
ISSN
1225-8687
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5483/BMBRep.2010.43.12.830
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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