Improving Pb2+ detection using DNAzyme-based fluorescence sensors by pairing fluorescence donors with gold nanoparticles

Cited 66 time in scopus
Metadata Downloads
Title
Improving Pb2+ detection using DNAzyme-based fluorescence sensors by pairing fluorescence donors with gold nanoparticles
Author(s)
Joong Hyun Kim; S H Han; Bong Hyun Chung
Bibliographic Citation
Biosensors & Bioelectronics, vol. 26, no. 5, pp. 2125-2129
Publication Year
2011
Abstract
For previously reported fluorescence Pb 2+ sensors, DNAzymes have lead to a significant increase in Pb 2+ detecting sensitivity and specificity. However, these sensors suffer from incomplete fluorescence quenching and require additional steps for annealing DNAzymes and substrates as well as for removing the uncoupled substrates. In this study, we successfully overcome these issues by immobilizing the substrate nucleic acids on gold nanoparticles through thiol linkages. The immobilization of the substrate molecules to the gold nanoparticles lead to almost-complete fluorescence quenching and fast Pb 2+ detection, without altering the Pb 2+ specificity of the DNAzymes. After optimizing the concentration of DNAzymes, reaction time and pH, we could detect Pb 2+ as low as 5nM within 20min without the preliminary and the post treatments. Considering the multi-color-fluorescence quenching capability of gold nanoparticles and the to-be-developed functional nucleic acids for other metal ions, this study could extend the application of DNAzymes to the detection of multiple heavy metal ions.
Keyword
DNAzymeFluorescence energy transferGold nanoparticlesLeadMultiplexingSensor
ISSN
0956-5663
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bios.2010.09.018
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.