A DNA microarray for identification of selected Korean birds based on mitochondrial cytochrome c oxidase i gene sequences

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Title
A DNA microarray for identification of selected Korean birds based on mitochondrial cytochrome c oxidase i gene sequences
Author(s)
I H Chung; Hye Sook Yoo; J Y Eah; H K Yoon; J W Jung; S Y Hwang; C B Kim
Bibliographic Citation
Molecules and Cells, vol. 30, no. 4, pp. 295-301
Publication Year
2010
Abstract
DNA barcoding with the gene encoding cytochrome c oxidase I (COI) in the mitochondrial genome has been proposed as a standard marker to identify and discover animal species. Some migratory wild birds are suspected of transmitting avian influenza and pose a threat to aircraft safety because of bird strikes. We have previously reported the COI gene sequences of 92 Korean bird species. In the present study, we developed a DNA microarray to identify 17 selected bird species on the basis of nucleotide diversity. We designed and synthesized 19 specific oligonucleotide probes; these probes were arrayed on a silylated glass slide. The length of the probes was 19-24 bps. The COI sequences amplified from the tissues of the selected birds were labeled with a fluorescent probe for microarray hybridization, and unique hybridization patterns were detected for each selected species. These patterns may be considered diagnostic patterns for species identification. This microarray system will provide a sensitive and a high-throughput method for identification of Korean birds. ⓒ 2010 The Korean Society for Molecular and Cellular Biology and Springer Netherlands.
Keyword
birdscytochrome c oxidase IDNA barcodingKoreamicroarraymolecular probes
ISSN
1016-8478
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.1007/s10059-010-0118-8
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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