Harvest of Scenedesmus sp. with bioflocculant and reuse of culture medium for subsequent high-density cultures

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Title
Harvest of Scenedesmus sp. with bioflocculant and reuse of culture medium for subsequent high-density cultures
Author(s)
D G Kim; Hyun Joon La; Chi-Yong Ahn; Y H Park; Hee-Mock Oh
Bibliographic Citation
Bioresource Technology, vol. 102, no. 3, pp. 3163-3168
Publication Year
2011
Abstract
The optimal flocculating conditions for harvesting high-density cultures of Scenedesmus sp. were investigated using inorganic coagulants and the bioflocculant produced by Paenibacillus polymyxa AM49. The flocculated medium as nutrients for subsequent algal cultivation was also tested. Consecutive treatment with 8.5mM CaCl2 and 0.2mM FeCl3 as coagulants and 1% bioflocculant from the culture broth of P. polymyxa AM49 showed the highest flocculating activity of up to 95% for high density algal cultures. The medium flocculated with the coagulants and bioflocculant showed less than 8% decrease in the growth yield in the subsequent algal cultivation. Furthermore, a 20% or 50% fresh BG11 medium supplement allowed the flocculated medium to maintain a high growth yield in subsequent algal cultivation. These results suggest that the flocculation method presented here is efficient and bio-friendly, and allows the reuse of the flocculated medium, thereby contributing to the economic cultivation and harvest of microalgae.
Keyword
BioflocculantFlocculationHarvestReuse of mediumScenedesmus sp.
ISSN
0960-8524
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.biortech.2010.10.108
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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