1,3-Propandiol production by engineered Hansenula polymorpha expressing dha genes from Klebsiella pneumoniae = 재조합 효모를 이용한 1,3-프로판디올의 제조

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Title
1,3-Propandiol production by engineered Hansenula polymorpha expressing dha genes from Klebsiella pneumoniae = 재조합 효모를 이용한 1,3-프로판디올의 제조
Author(s)
Won Kyung Hong; Chul Ho KimSun Yeon Heo; L H Luo; Baek Rock Oh; D Rairakhwada; Jeong-Woo Seo
Bibliographic Citation
Bioprocess and Biosystems Engineering, vol. 34, no. 2, pp. 231-236
Publication Year
2011
Abstract
Currently, 1,3-propanediol (1,3-PD) is an important chemical widely used in polymer production, but its availability is being restricted owing to its expensive chemical synthesis. A methylotrophic yeast Hansenula polymorpha was engineered by expression of dhaB1, dhaB2, dhaB3, dhaBRA1 and dhaBRA2 encoding glycerol dehydratase complex and dhaT encoding 1,3-PD oxidoreductase from Klebsiella pneumoniae under direction of promoter of glyceraldehyde-3 phosphate dehydrogenase (GAPDH). The engineered recombinant yeast strain can produce 1,3-PD from glucose (2.4 g L-1) as well as glycerol (0.8 g L-1), which might lead to a safe and cost-effective method for industrial production of 1,3-PD from various biomass resources.
Keyword
1,3-Propandiol1,3-Propanediol oxidoreductaseGlycerol dehydrataseHansenula polymorphaKlebsiella pneumoniae
ISSN
1615-7591
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00449-010-0465-z
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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