Efficient production of ethanol from crude glycerol by a Klebsiella pneumoniae mutant strain = Klebsiella pneumoniae 돌연변이 균주를 이용한 폐글리세롤로부터 에탄올 생산

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Title
Efficient production of ethanol from crude glycerol by a Klebsiella pneumoniae mutant strain = Klebsiella pneumoniae 돌연변이 균주를 이용한 폐글리세롤로부터 에탄올 생산
Author(s)
Baek Rock OhJeong-Woo SeoSun Yeon Heo; Won Kyung Hong; L H Luo; M H Joe; D H Park; Chul Ho Kim
Bibliographic Citation
Bioresource Technology, vol. 102, no. 4, pp. 3918-3922
Publication Year
2011
Abstract
A mutant strain of Klebsiella pneumoniae, termed GEM167, was obtained by γ irradiation, in which glycerol metabolism was dramatically affected on exposure to γ rays. Levels of metabolites of the glycerol reductive pathway, 1,3-propanediol (1,3-PD) and 3-hydroxypropionic acid (3-HP), were decreased in the GEM167 strain compared to a control strain, whereas the levels of metabolites derived from the oxidative pathway, 2,3-butanediol (2,3-BD), ethanol, lactate, and succinate, were increased. Notably, ethanol production from glycerol was greatly enhanced upon fermentation by the mutant strain, to a maximum production level of 21.5. g/l, with a productivity of 0.93. g/l/h. Ethanol production level was further improved to 25.0. g/l upon overexpression of Zymomonas mobilis pdc and adhII genes encoding pyruvate decarboxylase (Pdc) and aldehyde dehydrogenase (Adh), respectively in the mutant strain GEM167.
Keyword
Ethanol productionFermentationGlycerolKlebsiella pneumoniaeMutant
ISSN
0960-8524
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.biortech.2010.12.007
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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