Development of a fluorescent microsphere immunoassay for cystatin B (CSTB) in serum of patients with hepatocellular carcinoma

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Title
Development of a fluorescent microsphere immunoassay for cystatin B (CSTB) in serum of patients with hepatocellular carcinoma
Author(s)
N Y Ji; Yun Hee Kang; M Y Park; Chung Il Lee; M K Kim; D G Kim; J W Kim; Eun Young Song
Bibliographic Citation
Clinical Chemistry and Laboratory Medicine, vol. 49, no. 1, pp. 151-155
Publication Year
2011
Abstract
Background: Serum cystatin B (CSTB) concentrations have been reported to be increased in patients with hepatocellular carcinoma compared to concentrations seen in normal subjects. In this study, we developed a "fluorescent microsphere immunoassay" (FMI) capable of specifically detecting CSTB in serum. Methods: The FMI used a microparticle conjugated polyclonal antibody to CSTB and biotinylated monoclonal antibody as capture protein and probe protein, respectively. The results were obtained using the Bio-Plex 200 system. Results: The dose-response relationship between CSTB and fluorescent intensity showed linearity in the range 0-1000 pg/mL and 7 pg/mL, sensitivity lower than 11.2 pg/mL. This result revealed that the FMI system was more sensitive than enzyme-linked immunoassay (ELISA). Additionally, the FMI system used smaller sample volumes compared to ELISA. Conclusions: We measured CSTB with both the FMI and an ELISA procedure and compared the two methods. The CSTB concentrations in serum specimens as measured with the FMI assay system were similar to those measured with ELISA. Thus, the new FMI using the Bio-Plex system may be useful for detection of CSTB in human serum. -------------------------------------------------------------------------------- Reaxys Database Information|
Keyword
SerumCystatin BELISAFluorescent microsphere immunoassayHepatocellular carcinoma
ISSN
1434-6621
Publisher
Walter De Gruyter Gmbh
DOI
http://dx.doi.org/10.1515/CCLM.2011.008
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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