Taxonomic characterization and UV/VIS analysis of antagonistic marine actinomycete isolated from South West Coast of South Korea

Abstract

A total of 68 actinomycetes colonies were isolated from near sea shore marine environment locations of Bigeum island, South West coast of South Korea. Among them, 44 isolates were morphologically distinct on the basis of spore mass colour, reverse side colour, aerial and substrate mycelia formation and production of diffusible pigment. The majority of these isolates were assigned to the genus Streptomyces of which 23 isolates had antimicrobial activity; with one isolate showing broad spectrum of antimicrobial was on the basis of their morphological, physiological and biochemical properties. Phylogenetic analysis of a 16S rRNA gene sequence showed that strain Streptomyces hygroscopicus BD 49 forms a distinct clade within the Streptomyces 16S rRNA gene tree and closely related to Streptomyces lydicus strain BDUS 45. This strain possessed a broad spectrum of antimicrobial activity against Gram-positive, Gram-negative bacteria and fungi. The UV spectra of the active compounds in ethyl acetate showed peaks at 217 and 221 nm. The bioactive region was detected on the TLC plate (R f 0.70).