O-chain structure from the lipopolysaccharide of the human pathogen Halomonas stevensii strain S18214

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dc.contributor.authorG Pierettia-
dc.contributor.authorS Carilloa-
dc.contributor.authorKang-Kyu Kim-
dc.contributor.authorKeun Chul Lee-
dc.contributor.authorJung-Sook Lee-
dc.contributor.authorR Lanzettaa-
dc.contributor.authorM Parrillia-
dc.contributor.authorM M Corsaroa-
dc.date.accessioned2017-04-19T09:21:24Z-
dc.date.available2017-04-19T09:21:24Z-
dc.date.issued2011-
dc.identifier.issn00086215-
dc.identifier.uri10.1016/j.carres.2010.11.023ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9974-
dc.description.abstractHalomonas stevensii is a Gram-negative, pathogenic, moderately halophilic bacterium isolated from the blood of a renal care patient. It optimally grows at 30-35 °C at pH 8-9 and at a sea salt concentration ranging from 3.0% to 7.5%. Gram-negative bacterial infections are closely associated with the presence of the lipopolysaccharides (LPSs) on the outer membrane. These molecules consist of three regions covalently linked: the glycolipid (lipid A), the oligosaccharide region (core region), and the O-specific polysaccharide (O-chain, O-antigen). O-antigen seems to play an important role in the colonization step (adherence) and the ability to bypass host defense mechanisms. For this reason the structure elucidation of the O-chain repeating unit is important to improve knowledge about the role of LPS in the host-pathogen interaction. In this paper, we report the complete structure of the O-chain from the LPS of H. stevensii. The bacterial cells were cultivated and LPS was extracted by the PCP (phenol-chloroform-petroleum ether) method. After mild acid hydrolysis, the lipid A was removed by centrifugation and the obtained polysaccharide was analyzed by means of chemical analysis and one- and two-dimensional NMR spectroscopy giving the following structure-
dc.publisherElsevier-
dc.titleO-chain structure from the lipopolysaccharide of the human pathogen Halomonas stevensii strain S18214-
dc.title.alternativeO-chain structure from the lipopolysaccharide of the human pathogen Halomonas stevensii strain S18214-
dc.typeArticle-
dc.citation.titleCarbohydrate Research-
dc.citation.number2-
dc.citation.endPage365-
dc.citation.startPage362-
dc.citation.volume346-
dc.contributor.affiliatedAuthorKeun Chul Lee-
dc.contributor.affiliatedAuthorJung-Sook Lee-
dc.contributor.alternativeNamePierettia-
dc.contributor.alternativeNameCarilloa-
dc.contributor.alternativeName김광규-
dc.contributor.alternativeName이근철-
dc.contributor.alternativeName이정숙-
dc.contributor.alternativeNameLanzettaa-
dc.contributor.alternativeNameParrillia-
dc.contributor.alternativeNameCorsaroa-
dc.identifier.bibliographicCitationCarbohydrate Research, vol. 346, no. 2, pp. 362-365-
dc.identifier.doi10.1016/j.carres.2010.11.023-
dc.subject.keywordHalomonas-
dc.subject.keywordLipopolysaccharide-
dc.subject.keywordNMR spectroscopy-
dc.subject.keywordStructure elucidation-
dc.subject.localHalomonas-
dc.subject.localLipopolysaccharide-
dc.subject.localNMR spectroscopy-
dc.subject.localStructure elucidation-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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