DC Field | Value | Language |
---|---|---|
dc.contributor.author | G Pierettia | - |
dc.contributor.author | S Carilloa | - |
dc.contributor.author | Kang-Kyu Kim | - |
dc.contributor.author | Keun Chul Lee | - |
dc.contributor.author | Jung-Sook Lee | - |
dc.contributor.author | R Lanzettaa | - |
dc.contributor.author | M Parrillia | - |
dc.contributor.author | M M Corsaroa | - |
dc.date.accessioned | 2017-04-19T09:21:24Z | - |
dc.date.available | 2017-04-19T09:21:24Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0008-6215 | - |
dc.identifier.uri | 10.1016/j.carres.2010.11.023 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/9974 | - |
dc.description.abstract | Halomonas stevensii is a Gram-negative, pathogenic, moderately halophilic bacterium isolated from the blood of a renal care patient. It optimally grows at 30-35 °C at pH 8-9 and at a sea salt concentration ranging from 3.0% to 7.5%. Gram-negative bacterial infections are closely associated with the presence of the lipopolysaccharides (LPSs) on the outer membrane. These molecules consist of three regions covalently linked: the glycolipid (lipid A), the oligosaccharide region (core region), and the O-specific polysaccharide (O-chain, O-antigen). O-antigen seems to play an important role in the colonization step (adherence) and the ability to bypass host defense mechanisms. For this reason the structure elucidation of the O-chain repeating unit is important to improve knowledge about the role of LPS in the host-pathogen interaction. In this paper, we report the complete structure of the O-chain from the LPS of H. stevensii. The bacterial cells were cultivated and LPS was extracted by the PCP (phenol-chloroform-petroleum ether) method. After mild acid hydrolysis, the lipid A was removed by centrifugation and the obtained polysaccharide was analyzed by means of chemical analysis and one- and two-dimensional NMR spectroscopy giving the following structure | - |
dc.publisher | Elsevier | - |
dc.title | O-chain structure from the lipopolysaccharide of the human pathogen Halomonas stevensii strain S18214 | - |
dc.title.alternative | O-chain structure from the lipopolysaccharide of the human pathogen Halomonas stevensii strain S18214 | - |
dc.type | Article | - |
dc.citation.title | Carbohydrate Research | - |
dc.citation.number | 2 | - |
dc.citation.endPage | 365 | - |
dc.citation.startPage | 362 | - |
dc.citation.volume | 346 | - |
dc.contributor.affiliatedAuthor | Kang-Kyu Kim | - |
dc.contributor.affiliatedAuthor | Keun Chul Lee | - |
dc.contributor.affiliatedAuthor | Jung-Sook Lee | - |
dc.contributor.alternativeName | Pierettia | - |
dc.contributor.alternativeName | Carilloa | - |
dc.contributor.alternativeName | 김광규 | - |
dc.contributor.alternativeName | 이근철 | - |
dc.contributor.alternativeName | 이정숙 | - |
dc.contributor.alternativeName | Lanzettaa | - |
dc.contributor.alternativeName | Parrillia | - |
dc.contributor.alternativeName | Corsaroa | - |
dc.identifier.bibliographicCitation | Carbohydrate Research, vol. 346, no. 2, pp. 362-365 | - |
dc.identifier.doi | 10.1016/j.carres.2010.11.023 | - |
dc.subject.keyword | Halomonas | - |
dc.subject.keyword | Lipopolysaccharide | - |
dc.subject.keyword | NMR spectroscopy | - |
dc.subject.keyword | Structure elucidation | - |
dc.subject.local | Halomonas | - |
dc.subject.local | lipopolysaccharide (LPS) | - |
dc.subject.local | Lipopolysaccharide | - |
dc.subject.local | lipopolysaccharide | - |
dc.subject.local | Lipopolysaccharide (LPS) | - |
dc.subject.local | Lipopolysaccharides | - |
dc.subject.local | NMR spectroscopy | - |
dc.subject.local | structure elucidation | - |
dc.subject.local | Structure elucidation | - |
dc.description.journalClass | Y | - |
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