Analysis of microbial community during the anaerobic dechlorination of PCE/TCE by DGGE

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Title
Analysis of microbial community during the anaerobic dechlorination of PCE/TCE by DGGE
Author(s)
Byung-Hyuk Kim; Dae-Hyun Cho; Y Sung; Chi-Yong Ahn; Byung Dae Yoon; S C Koh; Hee-Mock OhHee-Sik Kim
Bibliographic Citation
Korean Journal of (Applied) Microbiology & Biotechnology, vol. 38, no. 4, pp. 448-454
Publication Year
2010
Abstract
This study investigated the effect of PCE and TCE as electron acceptors on the bacterial composition of dechlorinating communities. The enrichment cultures reductively dechlorinating PCE and TCE were developed from three environment samples using acetate as electron donor. The cultures were prepared by sequential enrichment, which was seeded with sediment and dredged soil. Denatured gradient gel electrophresis (DGGE) of 16S rRNA gene fragment was used to compare the microbial communities of these three enrichment cultures. After incubation for 4 weeks, the removal efficiencies of PCE and TCE were highest from Yeocheon site (87.37% and 84.46%, respectively). PCE and TCE as electron acceptors affected the bacterial diversity and community profiles in the enrichment cultures. DGGE analysis showed that the dominant bacteria in PCE and TCE enrichment were belonged to Clostridium sp., Desulfotomaculum sp., and uncultured bacteria.
Keyword
BioremediationDGGEMicrobial communityPCETCE
ISSN
0257-2389
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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