Dendritic cell activation by glucan isolated from Umbilicaria esculenta

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Title
Dendritic cell activation by glucan isolated from Umbilicaria esculenta
Author(s)
H S Kim; J Y Kim; H K Lee; M S Kim; S R Lee; Jong Soon Kang; Hwan Mook Kim; K A Lee; J T Hong; Y Kim; S B Han
Bibliographic Citation
Immune Network, vol. 10, no. 6, pp. 188-197
Publication Year
2010
Abstract
Background: Lichen-derived glucans have been known to stimulate the functions of immune cells. However, immunostimulatory activity of glucan obtained from edible lichen, Umbilicaria esculenta, has not been reported. Thus we evaluated the phenotype and functional maturation of dendritic cells (DCs) following treatment of extracted glucan (PUE). Methods: The phenotypic and functional maturation of PUE-treated DCs was assessed by flow cytometric analysis and cytokine production, respectively. PUE-treated DCs was also used for mixed leukocyte reaction to evaluate T cell-priming capacity. Finally we detected the activation of MAPK and NF-κB by immunoblot. Results: Phenotypic maturation of DCs was shown by the elevated expressions of CD40, CD80, CD86, and MHC class I/II molecules. Functional activation of DCs was proved by increased cytokine production of IL-12, IL-1β, TNF-α, and IFN-α/β, decreased endocytosis, and enhanced proliferation of allogenic T cells. Polymyxin B, specific inhibitor of lipopolysaccharide (LPS), did not affect PUE activity, which suggested that PUE was free of LPS contamination. As a mechanism of action, PUE increased phosphorylation of ERK, JNK, and p38 MAPKs, and enhanced nuclear translocation of NF-κB p50/p65 in DCs. Conclusion: These results indicate that PUE induced DC maturation via MAPK and NF-κB signaling pathways.
Keyword
caria esculentaGlucanDendritic cellsMAPKsNF-κB
ISSN
I000-0135
Publisher
South Korea
DOI
http://dx.doi.org/10.4110/in.2010.10.6.188
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Laboratory Animal Resource Center > 1. Journal Articles
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