DC Field | Value | Language |
---|---|---|
dc.contributor.author | J Bae | - |
dc.contributor.author | E H Choi | - |
dc.contributor.author | Jae Gu Pan | - |
dc.date.accessioned | 2017-04-19T09:21:29Z | - |
dc.date.available | 2017-04-19T09:21:29Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0141-0229 | - |
dc.identifier.uri | 10.1016/j.enzmictec.2010.11.002 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/9989 | - |
dc.description.abstract | For enzymatic synthesis of octyl-β-d-galactopyranoside (octyl-gal) from lactose and n-octanol, Escherichia coli β-galactosidase (β-Gal) was expressed and displayed on the surfaces of Bacillus subtilis spores. The spore-displayed β-Gal was found to be stable when an amphiphilic 1,2-dimethoxyethane (DME) was used as a co-solvent; the transgalactosylation efficiency and octyl-gal conversion were optimal at 50% (v/v) DME. In addition, the product was maximally obtained from 100mM lactose in a phosphate buffer/n-octanol/DME (25/25/50, v/v) mixture. By increasing the agitation speed and the amount of spores displaying β-Gal, a yield of 33.7mM octyl-gal was obtained over 24h in a batch mode, which is much higher than in other octyl-gal bioconversion processes, such as those involving lipid-coating, reverse micelles, or whole cells. On the other hand, intermittent addition of spore-displayed β-Gal and/or lactose in the reaction medium had no effect on the octyl-gal yield. The synthesized octyl-gal was hydrolyzed by the spore-displayed β-Gal, and a high concentration of octyl-gal competitively inhibited the enzymes (Ki value of 10.8mM). In summary, we demonstrate that octyl-gal synthesis by spore-displayed β-Gal in non-aqueous medium can be significantly improved with the use of DME as a co-solvent. | - |
dc.publisher | Elsevier | - |
dc.title | Efficient synthesis of octyl-β-d-galactopyranoside by Bacillus spore-displayed β-galactosidase using an amphiphilic 1,2-dimethoxyethane co-solvent | - |
dc.title.alternative | Efficient synthesis of octyl-β-d-galactopyranoside by Bacillus spore-displayed β-galactosidase using an amphiphilic 1,2-dimethoxyethane co-solvent | - |
dc.type | Article | - |
dc.citation.title | Enzyme and Microbial Technology | - |
dc.citation.number | 3 | - |
dc.citation.endPage | 238 | - |
dc.citation.startPage | 232 | - |
dc.citation.volume | 48 | - |
dc.contributor.affiliatedAuthor | Jae Gu Pan | - |
dc.contributor.alternativeName | 배정돈 | - |
dc.contributor.alternativeName | 최은화 | - |
dc.contributor.alternativeName | 반재구 | - |
dc.identifier.bibliographicCitation | Enzyme and Microbial Technology, vol. 48, no. 3, pp. 232-238 | - |
dc.identifier.doi | 10.1016/j.enzmictec.2010.11.002 | - |
dc.subject.keyword | β-Galactosidase | - |
dc.subject.keyword | Co-solvent | - |
dc.subject.keyword | Monoglyme | - |
dc.subject.keyword | Octyl galactoside | - |
dc.subject.keyword | Spore display | - |
dc.subject.keyword | Transgalactosylation | - |
dc.subject.local | β-galactosidase | - |
dc.subject.local | beta galactosidase | - |
dc.subject.local | ß-Galactosidase | - |
dc.subject.local | β-Galactosidase | - |
dc.subject.local | Co-solvent | - |
dc.subject.local | Monoglyme | - |
dc.subject.local | Octyl galactoside | - |
dc.subject.local | spore display | - |
dc.subject.local | Spore display | - |
dc.subject.local | Transgalactosylation | - |
dc.description.journalClass | Y | - |
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