Efficient synthesis of octyl-β-d-galactopyranoside by Bacillus spore-displayed β-galactosidase using an amphiphilic 1,2-dimethoxyethane co-solvent

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dc.contributor.authorJ Bae-
dc.contributor.authorE H Choi-
dc.contributor.authorJae Gu Pan-
dc.date.accessioned2017-04-19T09:21:29Z-
dc.date.available2017-04-19T09:21:29Z-
dc.date.issued2011-
dc.identifier.issn0141-0229-
dc.identifier.uri10.1016/j.enzmictec.2010.11.002ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/9989-
dc.description.abstractFor enzymatic synthesis of octyl-β-d-galactopyranoside (octyl-gal) from lactose and n-octanol, Escherichia coli β-galactosidase (β-Gal) was expressed and displayed on the surfaces of Bacillus subtilis spores. The spore-displayed β-Gal was found to be stable when an amphiphilic 1,2-dimethoxyethane (DME) was used as a co-solvent; the transgalactosylation efficiency and octyl-gal conversion were optimal at 50% (v/v) DME. In addition, the product was maximally obtained from 100mM lactose in a phosphate buffer/n-octanol/DME (25/25/50, v/v) mixture. By increasing the agitation speed and the amount of spores displaying β-Gal, a yield of 33.7mM octyl-gal was obtained over 24h in a batch mode, which is much higher than in other octyl-gal bioconversion processes, such as those involving lipid-coating, reverse micelles, or whole cells. On the other hand, intermittent addition of spore-displayed β-Gal and/or lactose in the reaction medium had no effect on the octyl-gal yield. The synthesized octyl-gal was hydrolyzed by the spore-displayed β-Gal, and a high concentration of octyl-gal competitively inhibited the enzymes (Ki value of 10.8mM). In summary, we demonstrate that octyl-gal synthesis by spore-displayed β-Gal in non-aqueous medium can be significantly improved with the use of DME as a co-solvent.-
dc.publisherElsevier-
dc.titleEfficient synthesis of octyl-β-d-galactopyranoside by Bacillus spore-displayed β-galactosidase using an amphiphilic 1,2-dimethoxyethane co-solvent-
dc.title.alternativeEfficient synthesis of octyl-β-d-galactopyranoside by Bacillus spore-displayed β-galactosidase using an amphiphilic 1,2-dimethoxyethane co-solvent-
dc.typeArticle-
dc.citation.titleEnzyme and Microbial Technology-
dc.citation.number3-
dc.citation.endPage238-
dc.citation.startPage232-
dc.citation.volume48-
dc.contributor.affiliatedAuthorJae Gu Pan-
dc.contributor.alternativeName배정돈-
dc.contributor.alternativeName최은화-
dc.contributor.alternativeName반재구-
dc.identifier.bibliographicCitationEnzyme and Microbial Technology, vol. 48, no. 3, pp. 232-238-
dc.identifier.doi10.1016/j.enzmictec.2010.11.002-
dc.subject.keywordβ-Galactosidase-
dc.subject.keywordCo-solvent-
dc.subject.keywordMonoglyme-
dc.subject.keywordOctyl galactoside-
dc.subject.keywordSpore display-
dc.subject.keywordTransgalactosylation-
dc.subject.localβ-galactosidase-
dc.subject.localbeta galactosidase-
dc.subject.localß-Galactosidase-
dc.subject.localβ-Galactosidase-
dc.subject.localCo-solvent-
dc.subject.localMonoglyme-
dc.subject.localOctyl galactoside-
dc.subject.localspore display-
dc.subject.localSpore display-
dc.subject.localTransgalactosylation-
dc.description.journalClassY-
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