A simple technique for consistently obtaining large single crystals of hen egg-white lysozyme in a concentration gradient of NiCl2

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Title
A simple technique for consistently obtaining large single crystals of hen egg-white lysozyme in a concentration gradient of NiCl2
Author(s)
E Magay; Tae-Sung Yoon
Bibliographic Citation
Journal of Applied Crystallography, vol. 44, no. 1, pp. 252-253
Publication Year
2011
Abstract
A simple protocol is reported for consistently obtaining large single crystals of hen egg-white lysozyme (HEWL) greater than 2 mm in the longest dimension. Comparative crystallization experiments with different sources of HEWL showed that the simple addition of glass capillaries, crystal hangers, reduced excessive nucleation and resulted in large single crystals regardless of the commercial source of HEWL.
Keyword
crystallizationhen egg-white lysozymeneutron diffraction
ISSN
0021-8898
Publisher
Int Union Crystallography
DOI
http://dx.doi.org/10.1107/S0021889810044936
Type
Article
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
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