Production of stable chloroplast-transformed plants in potato (Solanum tuberosum L.) = 안정적 감자 엽록체 형질전환 식물체 생산

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Title
Production of stable chloroplast-transformed plants in potato (Solanum tuberosum L.) = 안정적 감자 엽록체 형질전환 식물체 생산
Author(s)
Sung Ran MinWon Joong Jeong; Ji Hyun Park; Jae Il Lyu; Jeong Hee Lee; Kwang Hoon Oh; H J Chung; Jang Ryol Liu
Bibliographic Citation
Journal of Plant Biotechnology, vol. 38, no. 1, pp. 42-48
Publication Year
2011
Abstract
Chloroplast genetic engineering of higher plants offers several unique advantages compared with nuclear genome transformation, such as high levels of transgene expression, a lack of position effect due to site-specific transgene integration by homologous recombination, multigene engineering in a single transformation event and reducing risks of gene flow via pollen due to maternal inheritance. We established a reproducible chloroplast transformation system of potato using a tobacco specific plastid transformation vector, pCtVG (trnI-Prrn-aadA-mgfp-TpsbA-trnA). Stable transgene integration into chloroplast genomes and the homoplasmic state of the transgenome were confirmed by PCR and Southern blot analyses. Northern, immunoblot analysis, and GFP fluorescence imaging revealed high expression and accumulation of GFP in the plastids of potato leaves. This system would provide new opportunities for genetic improvement and mass production of value added foreign proteins in this crop.
ISSN
1229-2818
Publisher
South Korea
DOI
http://dx.doi.org/10.5010/JPB.2011.38.1.042
Type
Article
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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