Redox regulation of the tumor suppressor PTEN by glutaredoxin 5 and Ycp4

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Title
Redox regulation of the tumor suppressor PTEN by glutaredoxin 5 and Ycp4
Author(s)
Y Kim; K O Chay; I Kim; Y B Song; T Y Kim; S J Han; Y Ahn; S H Cho; Kwang Lae Hoe; B W Ahn; W K Huh; S R Lee
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 407, no. 1, pp. 175-180
Publication Year
2011
Abstract
Human PTEN (phosphatase and tensin homolog deleted on chromosome 10; a phosphatidylinositol 3-phosphatase) expressed in Saccharomyces cerevisiae was oxidized in a time- and H2O2-concentration-dependent manner. Oxidized hPTEN was reduced by cellular reductants as in human cells. The reduction rate of oxidized hPTEN was monitored in S. cerevisiae mutants in which the genes involved in redox homeostasis had been disrupted. Reduction of hPTEN was delayed in each of S. cerevisiae grx5Δ and ycp4Δ mutants. Expression of Grx5 and Ycp4 in each of the mutants rescued the reduction rate of oxidized hPTEN. Furthermore, an in vitro assay revealed that the human Grx5/GSH system efficiently catalyzed the reduction of oxidized hPTEN. These results suggest that the reduction of oxidized hPTEN is regulated by Grx5 and Ycp4
Keyword
Glutaredoxin 5GlutathioneHydrogen peroxidePTENYcp4
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bbrc.2011.02.133
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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