Novel quantitative method for the degree of branching in dextran

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Title
Novel quantitative method for the degree of branching in dextran
Author(s)
Young Min Kim; A Kimura; D Kim
Bibliographic Citation
Food Science and Biotechnology, vol. 20, no. 2, pp. 537-541
Publication Year
2011
Abstract
A novel quantitative method for the determination of degree of branching in Leuconostoc mesenteroides B- 512F dextran was developed by using the combination of 3 dextran-degrading enzymes. First, Paenibacillus sp. endo-dextranase was randomly degraded B-512F dextran into linear or branched isomalto-oligosaccharides with various degree of polymerization (2-8). Second, Streptococcus mutans dextran glucosidase hydrolyzed linear or branched isomalto-oligosaccharides into glucose and branched isomalto-penta-saccharides. Third, the branched isomaltopenta- saccharide was degraded into glucose by using Bacteroides thetaimicron α-glucosidase. The number of branching points in B-512F dextran (5.42%) was determined by the difference in the amount of glucose in the reaction digest between BTGase-PDex and DGase-PDex treatments.
Keyword
Degree of branchingDextranEndo-dextranaseExo-dextranase
ISSN
1226-7708
Publisher
South Korea
DOI
http://dx.doi.org/10.1007/s10068-011-0075-9
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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