Decolorization of indigo carmine by laccase displayed on Bacillus subtilis spores

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Title
Decolorization of indigo carmine by laccase displayed on Bacillus subtilis spores
Author(s)
E A Cho; Ji-Young Seo; Dong-Woo Lee; Jae Gu Pan
Bibliographic Citation
Enzyme and Microbial Technology, vol. 49, no. 1, pp. 100-104
Publication Year
2011
Abstract
Blue multicopper oxidases, laccases displayed on the surface of Bacillus spores were used to decolorize a widely used textile dyestuff, indigo carmine. The laccase-encoding gene of Bacillus subtilis, cotA, was cloned and expressed in B. subtilis DB104, and the expressed enzyme was spontaneously localized on Bacillus spores. B. subtilis spores expressing laccase exhibited maximal activity for the oxidation of 2,2′-azino-bis (3-ethylthiazoline-6-sulfonate) (ABTS) at pH 4.0 and 80 °C, and for the decolorization of indigo carmine at pH 8.0 and 60 °C. The displayed enzyme retained 80% of its original activity after pre-treatment with organic solvents such as 50% acetonitrile and n-hexane for 2 h at 37 °C. The apparent Km of the enzyme displayed on spores was 443 ± 124 μM for ABTS with a Vmax of 150 ± 16 U/mg spores. Notably, 1 mg of spores displaying B. subtilis laccase (3.4 × 102 U for ABTS as a substrate) decolorized 44.6 μg indigo carmine in 2 h. The spore reactor (0.5 g of spores corresponding to 1.7 × 105 U in 50 mL) in a consecutive batch recycling mode decolorized 223 mg indigo carmine/L to completion within 42 h at pH 8.0 and 60 °C. These results suggest that laccase displayed on B. subtilis spores can serve as a powerful environmental tool for the treatment of textile dye effluent.
Keyword
Bacillus subtilisDecolorizationIndigo carmineLaccaseSpore display
ISSN
0141-0229
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.enzmictec.2011.03.005
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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